食品科学2025，Vol.46Issue(3)：27-35,9.DOI:10.7506/spkx1002-6630-20240730-292
秦川牛宰后成熟过程中细胞自噬与肉色变化的相关性
Correlation between Cellular Autophagy and Color Changes in Qinchuan Cattle Meat during Postmortem Aging
摘要
Abstract
In order to investigate the occurrence of cellular autophagy and its effect on the meat color of Qinchuan cattle during postmortem aging,the changes in the meat color,relative myoglobin content,metmyoglobin reductase activity(MRA),beclin1,microtubule-associated protein light chain 3-II(LC3-II),autophagy-related protein 7(ATG7),sequestosome 1(p62),caspase 8,mitochondrial membrane potential(MMP),reactive oxygen species(ROS)levels of the longissimus dorsi muscle of Qinchuan cattle were determined at different time points during postmortem aging at 4℃.It was found that as the aging time was prolonged,L* and a* values as well as beclin1,LC3-II and ATG7 protein expressions initially increased and subsequently decreased,caspase 8,p62 and ROS showed an opposite trend;the relative contents of deoxymyoglobin(DeoMb)and oxymyoglobin(OxyMb),MMP and MRA decreased,whereas b* value and the relative content of metmyoglobin(MetMb)increased.Moreover,the key proteins related to cellular autophagy were expressed to different degrees during the postmortem process,and muscle cells exhibited autophagy,the strongest autophagic activity being observed at 96 h.Correlation analyses showed a highly significant correlation among the key proteins related to cellular autophagy(P<0.01),indicating that these proteins were closely related to each other in cellular activity.There were significant correlations between meat color parameters and cellular autophagy-related proteins and mitochondrial function at 0-96 h during the postmortem process(P<0.05).The above results showed that the changes in mitochondrial function and autophagy in myoblasts during the early postmortem period affect meat color,and beclin1,LC3-II,ATG7,p62,and caspase 8 can be used as biomarkers to predict the changes of meat color during the postmortem process.关键词
细胞自噬/线粒体功能/苄氯素1/微管相关蛋白1轻链3/肉色Key words
cellular autophagy/mitochondrial function/beclin1/microtubule-associated protein light chain 3-II/meat color分类
轻工业引用本文复制引用
梁燕群,司健芳,马萌,高爽,李亚蕾,罗瑞明..秦川牛宰后成熟过程中细胞自噬与肉色变化的相关性[J].食品科学,2025,46(3):27-35,9.基金项目
国家自然科学基金地区科学基金项目(32160535) （32160535）
