食品科学2025，Vol.46Issue(3)：90-101,12.DOI:10.7506/spkx1002-6630-20240520-165
枯草芽孢杆菌JZXJ-7对副溶血性弧菌感染小鼠肠道功能及菌群的影响
Effect of Bacillus subtilis JZXJ-7,a Strain Isolated from Shrimp Paste,on Colonic Function and Microbial Community in Mice Infected with Vibrio parahaemolyticus
摘要
Abstract
The protective effect of Bacillus subtilis JZXJ-7(JZXJ-7)on Vibrio parahaemolyticus(Vp)infected mice was investigated.Totally 28 male C57BL/6J mice were randomly divided into four groups:a Vp infection model group(infected with the standard virulence strain ATCC33847),two JZXJ-7 intervention groups(administered at low(106 CFU)and high(108 CFU)doses by gavage),and a control group.The control and model groups were administered with an equal volume of normal saline.Disease activity index(DAI),Vp load in the intestine tract(including the ileum,cecum,and colon),colonic pathological alterations,colonic cytokine contents,oxidative stress resistance,and changes in intestinal microbial community composition were detected in all groups.The results showed that the protective effect of JZXJ-7 was better at the high dose than at the low dose.High-dose JZXJ-7 significantly reduced the DAI and intestinal Vp load in Vp infected mice,alleviated inflammatory cell infiltration and collagen deposition,and increased the number of intestinal epithelial goblet cells(P<0.05).Moreover,high-dose JZXJ-7 significantly inhibited cytokine-mediated inflammation,increased antioxidant enzyme activities and enhanced intestinal permeability.Furthermore,JZXJ-7 significantly increased(P<0.05)intestinal microbial diversity,partially reversing intestinal flora disturbance,enriching Akkermansia,Bifidobacterium,Clostridium and other beneficial bacteria,and thus resulting in the restoration of intestinal microecological homeostasis.In summary,JZXJ-7 can effectively alleviate colonic pathological damage in Vp infected mice and maintain intestinal functions,and the underlying mechanism may be related to its regulatory effect on the intestinal microbiota.关键词
枯草芽孢杆菌JZXJ-7/副溶血性弧菌/感染/肠道微生态/保护作用Key words
Bacillus subtilis JZXJ-7/Vibrio parahaemolyticus/infection/intestinal microecology/protective effects分类
轻工纺织引用本文复制引用
周奎,王润东,宫梦学,牟景远,邓义佳,李学鹏,张宇昊,励建荣..枯草芽孢杆菌JZXJ-7对副溶血性弧菌感染小鼠肠道功能及菌群的影响[J].食品科学,2025,46(3):90-101,12.基金项目
国家自然科学基金青年科学基金项目(32202134 （）
32201929) （）
2025年广东省自然科学基金面上项目(2025A1515011748) （2025A1515011748）
岭南师范学院高层次人才专项(ZL2415) （ZL2415）
广东省科技创新战略专项(2022A05035) （2022A05035）
