食品科学2025，Vol.46Issue(3)：222-229,8.DOI:10.7506/spkx1002-6630-20240718-183
金属有机骨架基固相萃取-高效液相色谱法检测水产品中5种氟喹诺酮类药物残留
Determination of Five Fluoroquinolones in Aquatic Products by High Performance Liquid Chromatography with Solid Phase Extraction Using Metal-Organic Framework as Sorbent
摘要
Abstract
A novel metal-organic framework(MOF)material,UiO-66-NH2,was synthesized using a solvothermal method.Subsequently,a high performance liquid chromatography(HPLC)method with solid phase extraction using UiO-66-NH2 was established for the simultaneous detection of five fluoroquinolone(FQ)residues in aquatic products.The sample was extracted with acetonitrile in 1.0%formic acid,degreased with n-hexane,and concentrated by rotary evaporation.The extract was then redissolved in aqueous ammonia solution at pH 8.0.The FQs were enriched and purified with a solid phase extraction(SPE)column filled with 30 mg of UiO-66-NH2 using 6 mL of 20%acetic acid-methanol solution as the eluent.The collected eluate was blown to dryness under nitrogen,redissolved and detected by HPLC with a fluorescence detector.Under the established experimental conditions,good linear relationships were achieved for the five FQs in the concentration range of 0.005-0.50 mg/L,with determination coefficients(R2)of 0.999 9-1.0.The recovery rates ranged from 81.4%to 104.8%with relative standard deviation(RSD)of 1.29%-4.93%,and the limits of detection(LOD)were in the range of 0.21-2.05 μg/kg.The prepared SPE column could be reused up to five times,which was environmentally friendly and cost-effective.The established method had good accuracy,precision,reproducibility,and selectivity,and was suitable for the simultaneous detection of various trace FQ residues in aquatic products.关键词
金属有机骨架/固相萃取/高效液相色谱/水产品/氟喹诺酮类药物Key words
metal-organic framework/solid phase extraction/high performance liquid chromatography/aquatic products/fluoroquinolones分类
轻工纺织引用本文复制引用
牛灿杰,叶素丹,朱千聪,胡玉霞..金属有机骨架基固相萃取-高效液相色谱法检测水产品中5种氟喹诺酮类药物残留[J].食品科学,2025,46(3):222-229,8.基金项目
浙江省基础公益研究计划项目(自然科学基金探索项目)(LTGC24C200003) （自然科学基金探索项目）
浙江经贸职业技术学院省属高校基本科研业务费专项(23SBYB01) （23SBYB01）
浙江省教育厅科研资助项目(Y202353372) （Y202353372）
教育部高等学校科学研究发展中心《虚拟仿真技术在职业教育教学中的创新应用》专项(ZJXF2022188) （ZJXF2022188）
