食品与发酵工业2025，Vol.51Issue(1)：26-31,6.DOI:10.13995/j.cnki.11-1802/ts.038661
分离纯化重组解脂耶氏酵母发酵液中虫草素的研究
Isolation and purification of cordycepin from the fermentation of engineering Yarrowia lipolytica
摘要
Abstract
Multiple studies have demonstrated that using Yarrowia lipolytica as a cell factory for cordycepin biosynthesis is an effective way to produce cordycepin.To obtain the separation and purification process,this study utilized macroporous adsorption resins with differ-ent specifications to purify cordycepin from recombinant Y.lipolytica fermentation broth by static and dynamic purification.Results showed that the recovery rate and purity of cordycepin could reach 87.67％and 65.82％by using NKA-Ⅱ macroporous adsorption resin with a cordycepin concentration of 3 mg/mL and pH of 9,loading flow rate of 3 BV/h,loading volume of 10 BV,wash flow rate of 2 BV/h,wash volume of 3 BV,and 30％ethanol for elution solution,with a flow rate of 3 BV/h and volume of 15 BV.This study aims to provide data references for the future large-scale production and preparation of cordycepin using recombinant Y.lipolytica.关键词
虫草素/解脂耶氏酵母/分离纯化/大孔吸附树脂/甘蔗糖蜜Key words
cordycepin/Yarrowia lipolytica/separation and purification/macroporous adsorption resin/molasses引用本文复制引用
宋丽萍,段希宇,王翀,卢向阳,田云..分离纯化重组解脂耶氏酵母发酵液中虫草素的研究[J].食品与发酵工业,2025,51(1):26-31,6.基金项目
国家自然科学基金项目(21808052) （21808052）
湖南省自然科学基金项目(2022JJ50022) （2022JJ50022）
湖南省科技人才托举工程-"小荷"科技人才专项项目(2023TJ-X39) （2023TJ-X39）
