食品与发酵工业2025，Vol.51Issue(1)：32-40,9.DOI:10.13995/j.cnki.11-1802/ts.038491
menD表达强度对枯草芽孢杆菌中维生素K2产量的影响
Effect of menD expression on vitamin K2 production in Bacillus subtilis
摘要
Abstract
Vitamin K2(MK)is an important natural compound,which plays an important role in the treatment of various diseases.To determine the role of menD in MK synthesis in Bacillus subtilis,the effects of the different expression levels of menD on bacterial growth,biofilm morphology,and MK production were detected by constructing BS168-ΔmenD,BS168-P43-menD and BS168-PxylA-menD.The ex-pression intensity of the menD was detected by western-blot and the content of MK was detected by HPLC.The experimental result showed that the BS168-ΔmenD strain failed to form biofilm and couldn't grow well when standard menadione wasn't added in the medium.The biofilm and the MK content of BS168-P43-menD and BS168-PxylA-menD were significantly different from the original strain BS168.During static fermentation,the biofilm biomass and wrinkle degree of BS168-P43-menD and BS168-PxylA-menD were greatly increased.The biofilm biomass of BS168-P43-menD and BS168-PxylA-menD were 1.36 and 1.59 times that of the original strain,respectively.The MK contents of BS168-P43-menD,BS168-PxylA-menD were 1.64 and 1.87 times that of the original strain,respectively.The maximum yield of MK was 39.27 mg/L in BS168-PxylA-menD.The results indicated that the expression level of menD was an important factor affecting the formation of biofilm and MK in B.subtilis.关键词
枯草芽孢杆菌/维生素K2/启动子/表达强度/菌株选育Key words
Bacillus subtilis/vitamin K2/promoter/expression intensity/breeding of strains引用本文复制引用
黄俊宝,刘艳,黄茜琳,陈宇,高旭丽,罗雅妮,陶伟,郭明雨,刘永圆,吴晶..menD表达强度对枯草芽孢杆菌中维生素K2产量的影响[J].食品与发酵工业,2025,51(1):32-40,9.基金项目
国家自然科学基金项目(32372295) （32372295）
安徽省高校杰出青年科研项目(2023AH020013) （2023AH020013）
安徽省大学生创新创业计划项目(202310363254) （202310363254）
