食品与发酵工业2025，Vol.51Issue(1)：41-46,6.DOI:10.13995/j.cnki.11-1802/ts.038635
疏棉状嗜热丝孢菌脂肪酶LipTL的酶学性质及脱胶应用研究
Expression and characterization of the lipase LipTL from Thermomyces lanuginosus and its application in oil degumming
摘要
Abstract
Enzymatic degumming technology has great potential application in oil refining industry.Phospholipases are the major en-zymes that applied in oil degumming.However,the existing phospholipases are unable to meet the requirements of industrial degumming processes in terms of substrate selectivity and temperature stability,which limits the large-scale application of phospholipases in the oil re-fining industry.Researches have revealed that the lipase(LipTL)from Thermomyces lanuginosus has ability of hydrolyzing phospholipids.In this study,this lipase LipTL gene was heterologously expressed in Escherichia coli,then the recombinant LipTL was purified and char-acterized,and its application in oil degumming was also studied.Sequence alignment exhibited that LipTL showed high similarity with phospholipase A1;SDS-PAGE revealed that the molecular weight of LipTL was approximately 30 kDa;The optimal temperature and pH for LipTL were 55 ℃ and 9.0 respectively,and it could maintain over 90％relative activity after 2 hours of treatment at 55 ℃.After 6 hours of degumming reaction,LipTL reduced the residual phosphorus content of soybean oil and peanut oil to 5.2 mg/kg and 4.9 mg/kg,re-spectively.This study confirms that the lipase LipTL can be applied to oil degumming,which not only provides a new enzyme source for enzymatic degumming,but also lays a good foundation for the subsequent modification of this enzyme.关键词
疏棉状嗜热丝孢菌/脂肪酶/酶法脱胶/同源建模/酶学性质Key words
Thermomyces lanuginosus/lipase/enzymatic degumming/homology modeling/enzymatic properties引用本文复制引用
邵化,单明明,王琼波,徐瑞敏,陈曦,魏涛..疏棉状嗜热丝孢菌脂肪酶LipTL的酶学性质及脱胶应用研究[J].食品与发酵工业,2025,51(1):41-46,6.基金项目
中原科技创新人才项目(224200510017) （224200510017）
河南省重点研发专项(231111111700) （231111111700）
