食品与发酵工业2025，Vol.51Issue(1)：174-181,8.DOI:10.13995/j.cnki.11-1802/ts.038181
阿魏酸对乙醇诱导的LO2肝细胞DNA损伤的修复机制研究
Study on the mechanism of ferulic acid in the repair of ethanol-induced DNA damage in LO2 liver cells
摘要
Abstract
Ferulic acid is a natural compound,and its effects on liver cells are not fully understood.The aim of this study was to in-vestigate the potential repair mechanism of ferulic acid at different concentrations on ethanol-induced DNA damage in LO2 hepatocytes.The effects of ferulic acid on the proliferation of LO2 hepatocytes were analyzed by using different concentrations of ferulic acid.Further,the effects of ethanol on LO2 cell cycle and the intervention of ferulic acid were studied.Western blot was used to detect the expression of p-H2AX,a marker of DNA damage,and the changes of reactive oxygen species levels and autophagy were detected by flow cytometry.The results showed that ferulic acid can promote the proliferation of LO2 cells at low concentration,and significantly reduce the expression of ethanol-induced p-H2AX and reactive oxygen species levels at medium concentration(0.25-2 mmol/L),and may promote autophagy.However,high concentrations of ferulic acid have shown toxic effects on cells,with potential inhibitory effects on DNA repair and autoph-agy.This study shows that ferulic acid can counteract ethanol-induced DNA damage in LO2 hepatocytes in a specific concentration range and may enhance the repair capacity of cells by promoting the autophagy mechanism.However,at high concentrations,ferulic acid may re-verse its protective effect and show cytotoxicity.关键词
阿魏酸/LO2肝细胞/DNA损伤修复/细胞周期/活性氧/细胞自噬/流式细胞仪Key words
ferulic acid/LO2 hepatocytes/DNA damage repair/cell cycle/reactive oxygen species/cell autophagy/flow cytometer引用本文复制引用
钟恒,陈杉彬,王德良,杨冬莹,温银萍,罗蕊琪,黄丹..阿魏酸对乙醇诱导的LO2肝细胞DNA损伤的修复机制研究[J].食品与发酵工业,2025,51(1):174-181,8.基金项目
中轻集团科技创新项目(ZQ2022JC-QN03) （ZQ2022JC-QN03）
