食品与发酵工业2025，Vol.51Issue(1)：182-188,7.DOI:10.13995/j.cnki.11-1802/ts.039541
梯度醇沉盐藻多糖结构及免疫调节活性分析
Analysis on structure and immunostimulatory activity of polysaccharides from Dunaliella salina by fractional precipitation
摘要
Abstract
Polysaccharide of different molecular weights from Dunaliella salina(polysaccharide of Dunaliella salina,PD)was pre-pared by 40％,60％and 80％of ethanol fractional precipitation,which were named PD-40,PD-60 and PD-80,respectively.The physi-cochemical properties such as HPGPC,FT-IR,monosaccharide composition,SEM,TGA and Congo red assay of the three polysaccharide components were analyzed,and in vitro immune activity was also evaluated in RAW 264.7 cell culture.Results indicated that when the ethanol concentration increased to 40％,60％and 80％(v/v),three PD components with decreasing molecular weight(PD-40＞PD-60＞PD-80)were produced sequentially,and there was no difference in structure or thermal stability based on the FT-IR and TGA analysis.Meanwhile,three PD components were all composed of 10 monosaccharide residues with different molar ratios,among them,the PD40 component had the largest content and molecular weight(Mw=2.31 × 107),accounting for 61.4％of the total PD mass,and also had the highest galactose(Gal)molar ratio and uronic acid content.Additionally,PD40 components could significantly enhance the production of NO and ROS as well as the phagocytic activity in RAW 264.7 cells.Ethanol fractional precipitation is an effective method for isolating immunoactive PD from D.salina while reducing solvent consumption.关键词
分级沉淀/盐藻/免疫活性/理化性质/单糖组成Key words
fractional precipitation/Dunaliella salina/immunostimulatory/physicochemical property/polysaccharide composition引用本文复制引用
张喜峰,张浩,刘霞,王兆丰,罗光宏..梯度醇沉盐藻多糖结构及免疫调节活性分析[J].食品与发酵工业,2025,51(1):182-188,7.基金项目
甘肃省科技计划项目(22JR11RG220,18JR2JG001) （22JR11RG220,18JR2JG001）
河西学院博士科研启动金项目(KYQD2023001) （KYQD2023001）
大学生创新创业训练计划项目(20221074021) （20221074021）
