食品与发酵工业2025，Vol.51Issue(1)：199-207,9.DOI:10.13995/j.cnki.11-1802/ts.038599
高压脉冲电场处理对中国对虾抗氧化活性和蛋白质特性的影响
Effects of high intensity pulsed electric field treatment on antioxidant activity and protein properties of Fenneropenaeus chinensis
摘要
Abstract
In order to investigate the effects of different high intensity pulsed electric field treatments on antioxidant activity and pro-tein properties of Fenneropenaeus chinensis,the total antioxidant capacity,total reducing power,DPPH radical scavenging activity,ferrous ion chelating capacity,and astaxanthin content of samples were measured before and after treatment with a pulsed electric field(10-20 kV/cm,5-15 min)to assess their antioxidant properties.The effects of the treatment on the amino acid content,soluble protein con-tent,and dielectric properties(1-10 GHz)were analyzed to investigate its impact on the protein properties of F.chinensis.The results indicated that high intensity pulsed electric field treatment could enhance the antioxidant capacity of shrimp meat.Under the condition of 20 kV/cm treatment for 15 min,the total antioxidant capacity,total reducing power,DPPH free radical scavenging activity,and ferrous ion chelating capacity of shrimp meat increased by 15.88％,36.51％,62.14％,and 43.52％,respectively.At electric field strengths of 15 kV/cm and 20 kV/cm,the astaxanthin content and free amino acid content increased gradually with increasing treatment time(P＜0.05).Moreover,the astaxanthin content increased by up to 1.61 times,and its variation was significantly correlated with the changes in the antioxidant activity of shrimp meat(P＜0.05).Compared to the untreated group,the ratio of essential amino acids to non-essential amino acids in shrimp meat significantly increased after pulsed electric field treatment(P＜0.05),and the dielectric constant gradually increased with the decrease in soluble protein content(P＜0.05).High intensity pulsed electric field treatment can enhance the antioxi-dant activity of shrimp meat,improve protein quality,and enhance amino acid utilization,providing new insights into the processing meth-ods and biological activity enhancement.关键词
高压脉冲电场/中国对虾/抗氧化活性/蛋白质特性/虾青素/氨基酸Key words
high intensity pulsed electric field/Fenneropenaeus chinensis/antioxidant activity/protein properties/astaxanthin/amino acid引用本文复制引用
杨晨宇,王肖园,邓云,陶宁萍,李立,邱伟强,李峰,金银哲..高压脉冲电场处理对中国对虾抗氧化活性和蛋白质特性的影响[J].食品与发酵工业,2025,51(1):199-207,9.基金项目
国家重点研发计划项目(2020YFD0900905) （2020YFD0900905）
