中国食品添加剂2025，Vol.36Issue(1)：18-24,7.DOI:10.19804/j.issn1006-2513.2025.1.004
在线凝胶渗透色谱-气质联用测定烟熏肉中7种N-亚硝胺类化合物的一测多评研究
QAMS of seven N-nitrosamines compounds in smoked meat by online gel permeation chromatography-gas chromatography-mass spectrometry
摘要
Abstract
In order to establish a quantitative analysis of multi-components by single marker (QAMS) method for the determination of seven N-nitrosamines in smoked meat using QuEChERS combined with online gel permeation chromatography (GPC) technology. Smoked meat samples were extracted with acetonitrile under an alkaline condition,and dehydrated with anhydrous magnesium sulfate and anhydrous sodium acetate. After purification using C18 and PSA,the samples underwent further purification through online gel permeation chromatography,followed by detection using mass spectrometry. Results indicated that the optimized QuEChERS method combined with online GPC effectively removed impurities from smoked meat samples,reducing matrix effects. The correction factors were as follows:?NDMA/NPYR=1.4073,?NMEA/NPYR=0.9491,?NDEA/NPYR=0.9433,?NDPA/NPYR=0.3822,?NDBA/NPYR=1.6247,?NPIP/NPYR=1.0263. The detection limits for the seven N-nitrosamine compounds ranged from 0.03 to 0.25 μg/kg. The recovery rates and precision for spiked samples were:NDMA 103.4％～106.7％ (RSD 2.4％～3.8％);NMEA 102.5％～107.6％ (RSD 2.6％～3.8％);NDEA 102.7％～108.1％ (RSD 2.5％～3.8％);NDPA 104.3％～106.4％ (RSD 1.8％～3.9％);NDBA 102.4％～108.3％ (RSD 1.8％～3.7％);NPIP 102.8％-106.8％ (RSD 2.2％～3.6％);NPYR 102.5％～107.8％ (RSD 2.1％～3.7％). This accurate and efficient method facilitated the rapid and simultaneous determination of seven N-nitrosamines compounds in large batches of smoked meat products.关键词
一测多评/N-亚硝胺类化合物/在线凝胶渗透色谱/气质联用/QuEChERSKey words
quantitative analysis of multi-components by single marker(QAMS)/N-nitrosamine compounds/online gel permeation chromatography/GC-MS/MS/QuEChERS分类
轻工纺织引用本文复制引用
吕谦,尹明月..在线凝胶渗透色谱-气质联用测定烟熏肉中7种N-亚硝胺类化合物的一测多评研究[J].中国食品添加剂,2025,36(1):18-24,7.基金项目
四川省药品监督管理局科技计划项目(编号:2021007) （编号:2021007）
