沈阳农业大学学报2025，Vol.56Issue(1)：1-8,8.DOI:10.3969/j.issn.1000-1700.2025.01.001
基于高密度遗传图谱发掘杂草稻早抽穗QTL
Exploring QTL for Early Heading of Weedy Rice Based on High-density Genetic Map
摘要
Abstract
[Objective]The heading date of rice(Oryza sativa L.)is a quantitative trait regulated by multiple genes,which affects the yield and quality of rice,and determines the planting area and distribution range,heading marks the transformation of rice from vegetative growth to reproductive growth.Weedy rice(Oryza sativa f.spontanea)as a kind of early heading germplasm resources,its heading date is often earlier than that of cultivar,while its genetic mechanism has not been fully elucidated.[Methods]In this study,a recombinant inbred line population consisting of 168 individuals was constructed based on early heading japonica weedy rice WR04-6 and late heading indica rice Qishanzhan.A high-density SNP genetic map was used to apply a inclusive composite interval mapping with additive effect model for QTL mapping at heading stage.[Results]Above the threshold line with a LOD score of 2.5,the only QTL regulating early heading of weedy rice was identified,containing 70 genes in the interval.Based on tissue-specific expression profile analysis and gene annotation,LOC_Os01g08700,also known as OsGI,was speculated as the target candidate gene.Subsequent verification and bioinformatics analysis showed significant differences between different haplotypes of OsGI,which located in the nucleus of the cell and exhibiting significant inter subspecies differentiation between indica and japonica rice.[Conclusion]In the heading regulation pathway of rice OsGI-Hd1-Hd3a/RFT1,Hd1 variation has been widely reported to regulate the heading stage and distribution of rice.However,this study found that the natural variation of the upstream circadian clock gene OsGI in weedy rice may affect its adaptive distribution.This study provides a new perspective on the molecular genetic regulation mechanism of early heading in weedy rice,and also contributes new a targets for the application of early heading gene resources in cultivated rice genetic improvement.关键词
抽穗期/杂草稻/遗传图谱/QTL定位/生物钟Key words
heading date/weedy rice/genetic map/QTL mapping/circadian clock分类
农业科技引用本文复制引用
李转,梁铖玮,于晓玉,韩雨晴,崔娟,赵雪,孙健,陈温福..基于高密度遗传图谱发掘杂草稻早抽穗QTL[J].沈阳农业大学学报,2025,56(1):1-8,8.基金项目
国家自然科学基金面上项目(32372107) （32372107）
