沈阳农业大学学报2025，Vol.56Issue(1)：29-39,11.DOI:10.3969/j.issn.1000-1700.2025.01.004
天女木兰PIN基因家族的鉴定及在种子萌发过程中的表达
Identification of PIN Genes Family and Their Expression During Seed Germination in Magnolia sieboldii K.Koch
摘要
Abstract
[Objective]Magnolia sieboldii K.Koch is a rare and endangered wild woody plant resource in China that exhibits ornamental,aromatic,and medicinal values,and has broad prospects for development and application.The asexual reproduction of this tree species is challenging,primarily due to the difficulty in sowing propagation.The seeds exhibit deep dormancy characteristics,and incomplete embryo development impedes seed germination and emergence,significantly constraining the development and application process.Auxin efflux carrier proteins,which are linked to seed development and germination,are encoded by the PIN gene family and carry auxin across the cell membrane.[Methods]By analyzing the structure and function of the PIN gene family,this study explores the transport regulation mechanism of auxin during seed after-ripening,providing a reference for in-depth research on the regulatory mechanisms of M.sieboldii K.Koch seed development.Using bioinformatics techniques and based on the genome data of M.sieboldii K.Koch,a genome-wide identification of the MsPINs gene family was conducted,studying the molecular structure,spatiotemporal expression during seed germination,and gene functions of the MsPINs gene.[Results]The results indicate:The M.sieboldii K.Koch genome has 22 MsPINs genes spread across 13 chromosomes,according to bioinformatics and expression research.Nine of these genes exhibit variable expression throughout the germination process.MsPIN1a～1c and MsPIN6 are upregulated during seed germination,MsPILS3 exhibits variable variations during the dormancy release process,and MsPIL3,MsPILS2,and MsPILS6 are upregulated during the dormancy release stage.The promoter sequence of MsPIN1b contains several MsARF5(Auxin Response Factor 5)recognitions,according to sequence analysis and yeast one-hybrid tests.[Conclusion]These findings suggest that M.sieboldii K.Koch seed growth and germination are mediated by members of the PIN gene family.关键词
天女木兰/生长素/MsPIN1b/表达模式/酵母单杂交Key words
Magnolia sieboldii K.Koch/auxin/MsPIN1b/expression pattern/yeast one hybrid分类
农业科技引用本文复制引用
梅梅,王斯彤,刘怡菲,虞家昕,张芷瑞,陆秀君..天女木兰PIN基因家族的鉴定及在种子萌发过程中的表达[J].沈阳农业大学学报,2025,56(1):29-39,11.基金项目
辽宁省博士科研启动基金计划项目(2022-BS-051) （2022-BS-051）
国家自然科学基金项目(32101508) （32101508）
辽宁省农业科学院院长基金项目(2022BS0704) （2022BS0704）
