现代食品科技2025，Vol.41Issue(1)：47-56,10.DOI:10.13982/j.mfst.1673-9078.2025.1.1415
蝉棒束孢C1菌丝体多糖抑制MAPK/NF-κB信号通路缓解LPS诱导的RAW264.7细胞炎症反应
Anti-inflammatory Effects of Polysaccharides from Isaria cidada Miq.C1 Mycelial through the MAPK/NF-κB Signaling Pathways in RAW264.7 cell induced by LPS
摘要
Abstract
The anti-inflammatory effects and mechanisms of the mycelial polysaccharide ISP-80 from the Isaria cicada Miquel C1 was investigated in a mouse monocyte-macrophage RAW264.7 inflammation model,induced by lipopolysaccharide(LPS).The polysaccharide(ISP-80)was obtained through hot water extraction from the Isaria cidada C1 mycelium.The toxic effects of ISP-80 on RAW264.7 cells were evaluated using the CCK-8 assay,and the impact of ISP-80 on the secretion of TNF-α,IL-1β,and IL-6 cytokines was measured using ELISA.Furthermore,the mechanism of ISP-80 was analyzed using western blotting to detect the relevant proteins in the MAPK/NF-κB pathways.The findings indicated that the extraction rate of ISP-80 was 10.42%and it was composed of three polysaccharide fractions,each containing Glc,Man,and Gal monosaccharides,in a molar ratio of 1.00:5.03:1.55.Adding ISP-80 at 0.01～1.00 mg/mL exerted no cytotoxic effects and promoted cell proliferation(P<0.000 1).The 0.25 mg/mL ISP-80 treatment significantly decreased the secretion of pro-inflammatory cytokines IL-1β(P<0.001)and IL-6(P<0.05),reaching 0.59-and 0.88-times those of the LPS group,respectively,and inhibited cell differentiation while restoring cell morphology.The anti-inflammatory action of ISP-80 acted via the MAPK/NF-κB pathways.ISP-80 inhibited the MAPK pathway by blocking p-p38 and p-JNK,which decreased by 0.20 and 0.41,respectively(P<0.001).Furthermore,the NF-κB pathway was inhibited by blocking IκBα and p65,which decreased by 0.43(P<0.01)and 0.24(P<0.001),respectively.These results indicate that ISP-80 can be a natural and safe anti-inflammatory agent for the prevention and treatment of inflammatory diseases.关键词
蝉棒束孢C1/多糖/抗炎作用/MAPK通路/RAW264.7巨噬细胞Key words
Isaria cidada Miquel/polysaccharide/anti-inflammatory effects/MAPK pathway/RAW264.7 macrophages引用本文复制引用
邵丽,牛珂欣,刘惠,蒋虹,贾庆文,马来记..蝉棒束孢C1菌丝体多糖抑制MAPK/NF-κB信号通路缓解LPS诱导的RAW264.7细胞炎症反应[J].现代食品科技,2025,41(1):47-56,10.基金项目
香料香精化妆品省部共建协同创新中心开放课题(1021ZK230016013) （1021ZK230016013）
