现代食品科技2025，Vol.41Issue(1)：57-64,8.DOI:10.13982/j.mfst.1673-9078.2025.1.1426
铁皮石斛毛兰素对人食管癌KYSE-150细胞的抑制作用
Inhibitory Effect of Erianin from Dendrobium officinale on Human Esophageal Cancer KYSE-150 Cells
摘要
Abstract
The inhibitory effect of erianin extracted from Dendrobium officinale on the growth of human esophageal cancer KYSE-150 cells was investigated.The CCK-8 kit,microscope,scratch test,and flow cytometry were used to determine the inhibitory effect,morphological changes,cell migration,cell apoptosis,and cycle arrest of erianin on the activity of KYSE-150 cells.Western blot analysis was used to detect the expression levels of Bax,Bcl-2,Vimentin,Akt,P-Akt,PI3K,P-PI3K,N-cadherin,and E-cadherin proteins.The results of cell experiments showed that the IC50 of erianin for inhibiting the growth of KYSE-150 cells at 24,48,and 72 h was 357.30,120.20,and 57.43 nmol/L,respectively.The cells exhibited reduced migration capability and demonstrated shrinkage and aggregation during growth.After treatment with 40,80,and 120 nmol/L for 24 h,the percentage of apoptosis was found to be 27.57%,35.38%,and 61.97%,respectively,compared with the control group.The proportion of cells in the G1 phase in the treatment group significantly decreased from 56.64%to 2.80%,whereas the proportion of cells in the G2 phase significantly increased from 16.82%to 86.98%.The western blot results showed that the expression of Bax and E-cadherin increased,whereas the expression of Bcl-2,Vimentin,P-Akt,PI3K,P-PI3K,and N-cadherin decreased,indicating that erianin has the potential to induce apoptosis and inhibit cell migration.In summary,erianin from D.officinale has the capability to inhibit cell migration through the inhibition of the EMT pathway and KYSE-150 cell proliferation and induce apoptosis via regulation of the PI3K/Akt signaling pathway.关键词
铁皮石斛/毛兰素/人食管癌KYSE-150细胞/PI3K/Akt信号通路Key words
Dendrobium officinale/erianin/human esophageal cancer KYSE-150 cells/PI3K/Akt signaling pathway引用本文复制引用
燕文柏,周俊含,甘菊丽,杜彦锋,张薇薇,赵庆俊,胡艺萍,罗纪红,邓晓东,赵志伟,魏玉颖,蒋璐遥,严阳..铁皮石斛毛兰素对人食管癌KYSE-150细胞的抑制作用[J].现代食品科技,2025,41(1):57-64,8.基金项目
四川中医药大健康产业发展与乡村振兴研究中心一般项目(DJKYB202302) （DJKYB202302）
成都医学院医院发展研究中心项目(YYFZ23001) （YYFZ23001）
成都医学院省级大学生创新创业训练计划项目(S202213705064) （S202213705064）
成都医学院国家级、省级大学生创新创业训练计划(202313705027) （202313705027）
成都医学院研究生科研创新基金一般项目(YCX2023-01-49) （YCX2023-01-49）
重大疾病靶点发现与蛋白药物研发四川省高校重点实验室项目(23LHZY04) （23LHZY04）
四川省中医药管理局中医药科研专项课题一般项目(2024MS232) （2024MS232）
