肉类研究2025，Vol.39Issue(2)：46-54,9.DOI:10.7506/rlyj1001-8123-20240902-228
微生物检测新技术在肉品中的应用研究进展
Application of New Microbial Detection Techniques in Meats:An Update
摘要
Abstract
Meat is a vital source of protein for consumers worldwide.Microbial spoilage not only leads to economic losses,but also may cause foodborne illnesses.Classical detection techniques are simple to operate but also have some drawbacks such as long detection time and low sensitivity,making them inadequate to meet the demands of modern food safety.With the application of new techniques,microbial detection has seen dramatic improvements in speed,sensitivity,and specificity.This review summaries the advantages and shortcomings of classical microbial detection methods,including culture,immunology,biochemical reaction,polymerase chain reaction(PCR),and fluorescence,and introduces the principles and advantages of new technologies such as high-throughput sequencing,digital PCR(dPCR),matrix-assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF MS),infrared spectroscopy,Raman spectroscopy,and hyperspectroscopy.Future prospects for their application in food safety testing are also explored,aiming to provide technical support for improving meat safety monitoring.关键词
肉品微生物/食品安全/经典检测技术/微生物检测新技术/应用Key words
meat microbiology/food safety/classical detection techniques/new microbial detection techniques/application分类
轻工纺织引用本文复制引用
熊颖涵,李重言,李若林,吴饶,周智威,孙宏虎,孙群..微生物检测新技术在肉品中的应用研究进展[J].肉类研究,2025,39(2):46-54,9.基金项目
国家自然科学基金面上项目(32470009) （32470009）
国家科技重大专项(2024ZD1000603) （2024ZD1000603）
