食品工业科技2025，Vol.46Issue(5)：81-90,10.DOI:10.13386/j.issn1002-0306.2024030318
分离纯化对银耳多糖结构及体外降脂活性的影响
Effects of Separation and Purification on the Structure and Lipid-lowering Activity in Vitro of Tremella fuciformis Polysaccharide
摘要
Abstract
In this study,Tremella fuciformis crude polysaccharide(TFP)was extracted using hot water extraction and refined to yield TFP-1 using a DEAE-52 cellulose column.This study evaluated the pure and unpurified polysaccharides'composition,structure,and in vitro hypolipidemic action.The total sugar content of TFP-1 grew to 78.19％,the uronic acid content increased to 31.39％,and the viscosity reduced to 108.33 mPa·s in comparison to TFP,according to the data.TFP had more linear macromolecules and a broader variety of molecular weights.Before and after purification,the composition of monosaccharides did not change,but the molar ratio did.Mannose,xylose,fucose,glucuronic acid,glucose,galactose,and glucosamine hydrochloride made up the monosaccharide composition of TFP and TFP-1.Their molar ratios were 0.51∶0.212∶0.182∶0.076∶0.013∶0.006∶0.001 and 0.552∶0.193∶0.132∶0.103∶0.014∶0.004∶0.003.Furthermore,the smooth,flat microscopic structure of the refined polvsaccharides eave wav to a roueh,fibrous structure.The results of in vitro lipid-lowering tests demonstrated that TFP had a considerably greater cholesterol binding rate(32.64％)at 10 mg/mL and pancreatic lipase inhibition rate(61.51％)at 15 mg/mL compared to TFP-1(P＜0.05).At 10 mg/mL,TFP-l's bile acid binding rate was 27.43％,substantially greater than TFP's(P＜0.05).This suggested that Tremella fuciformis polysaccharide's separation and purification altered some of its structural characteristics,which in turn had an impact on its hypolipidemic action.The findings offered a theoretical foundation for the creation of foods related to Tremella fuciformis polysaccharide that had the ability to decrease cholesterol.关键词
银耳多糖/多糖制备纯化/分子量/单糖组成/降脂活性Key words
Tremella fuciformis polysaccharide/polysaccharide preparation and purification/molecular weight/monosaccharide composition/lipid-lowering activity分类
轻工纺织引用本文复制引用
王一涵,谢清芸,滕建文,黄丽,夏宁..分离纯化对银耳多糖结构及体外降脂活性的影响[J].食品工业科技,2025,46(5):81-90,10.基金项目
广西科技重大专项(桂科AA20302012-3). （桂科AA20302012-3）
