食用菌学报2025，Vol.32Issue(1)：1-9,9.DOI:10.16488/j.cnki.1005-9873.2025.01.001
一株野生香菇与'申香215'孢子单核体杂交后代表型多样性分析
Phenotypic Diversity Analysis of Monokaryon Hybrids Between a Wild Lentinula edodes Strain and'Shenxiang 215'
摘要
Abstract
In order to explore the efficiency of using wild Lentinula edodes germplasm resource of Yunnan in cross breeding and the diversity of monokaryons of the elite variety'Shenxiang 215',a selected monokaryotic strain DH10 derived from a wild Yunnan L.edodes was pairwise crossed with 190 monokaryotic strains of'Shenxiang 215'respectively to generate a hybrid strain population.The hybrid strains were then evaluated for 11 categorical agronomic traits and 10 numerical agronomic traits,and analyzed for diversity.The results showed that 148 of the 190 hybrid strains developed fruiting bodies normally,and the diversity index of graded agronomic traits ranged from 0.071 to 1.386 with an average of 0.627.Compared with the other three mating types,the A2B3A4B4 mating type hybrid strains fruited with a high uniformity(diversity index 0.210).The stipes of the A2B2A4B4 and A3B2A4B4 mating types hybrid strains were evenly thick from top to bottom(diversity index 0).Hybrid strains with mating types A2B2A4B4 and A2B3A4B4 were easy to open pileus.The diversity index of numerical agronomic traits of the hybrid population ranged from 0.991 to 1.755,with an average of 1.548.Among them,stipe length showed the highest diversity index(1.755),and stipe diameter showed the lowest diversity index(0.991).The variation coefficient of all numerical agrinomic traits ranged from 16.55％to 73.52％with an average of 41.03％.Among them,the variation coefficient of number of fruiting bodies was the highest(73.52％),and that of stipe length was the lowest(16.55％).For the numerical agronomic traits,only the distribution of yield per bag fitted the normal distribution.The A2B3A4B4 mating type hybrid strains showed the greatest single fruiting body weight with heavy and thick pilei.The A3B2A4B4 mating type hybrid strains had a low stipe weight.The results provided a reference for utilization of wild germplasm resources and cross breeding research of L.edodes.关键词
香菇/野生种质/孢子单核体/交配型/多样性/农艺性状Key words
Lentinula edodes/wild germplasm/spore monokaryon/mating type/diversity/agronomic traits引用本文复制引用
徐蒋振,李洁,张帅,董浩然,邵阳浩,章炉军,张丹,宋春艳..一株野生香菇与'申香215'孢子单核体杂交后代表型多样性分析[J].食用菌学报,2025,32(1):1-9,9.基金项目
国家重点研发计划项目(2023YFD1201604) （2023YFD1201604）
江苏省重点研发计划现代农业项目(BE2022334) （BE2022334）
