食用菌学报2025，Vol.32Issue(1)：18-24,7.DOI:10.16488/j.cnki.1005-9873.2025.01.003
金针菇异质单核菌株培育
Preparation of Alloplasmic Monokaryotic Strains of Flammulina filiformis
摘要
Abstract
Using single-factor experiments,the effect of enzymolysis temperature,enzymolysis time,type of osmotic stabilizer,and incubation mode on protoplast yield of dikaryotic Flammulina filiformis strain W8-Y33 was studied.Through monokaryotic hybridization and microscopic examination,a monokaryotic strain named H33 was selected.It was compatible with the monokaryotic strain W8 but incompatible with the monokaryotic strain Y33.Then H33 was observed for colony morphology and analyzed for mitochondrial type.The results showed that the protoplast yield of W8-Y33 reached the highest(1.5×106 cells per milliliter)when mycelia cultured in shaking flasks for 7 d were enzymolyzed at 28 ℃ for 2 h with mannitol as the osmotic stabilizer.The selected monokaryotic strain H33 containing the cytoplasm of W8 exhibited significant differences in colony morphology compared with monokaryotic strains Y33 and W8.H33,W8 and W8-Y33 contained the same type of mitochondria,that was different from that of Y33.These results provided a reference for further research on cytoplasmic inheritance and variety improvement of F.filiformis.关键词
金针菇/核迁移/原生质体/异质单核菌株Key words
Flammulina filiformis/nuclear migration/protoplast/alloplasmic monokaryotic strain引用本文复制引用
贺渊倩,刘宁宁,刘慧馨,李华,刘靖宇..金针菇异质单核菌株培育[J].食用菌学报,2025,32(1):18-24,7.基金项目
山西省研究生科研创新项目(2024KY324) （2024KY324）
国家自然科学基金(32072647) （32072647）
优秀博士毕业生来晋工作奖励经费科研项目(晋财教[2022]102号) （晋财教[2022]102号）
山西省科技重大专项计划"揭榜挂帅"项目(202301140601015) （202301140601015）
山西省现代农业产业技术体系建设项目(SYJTX2022-03) （SYJTX2022-03）
