食用菌学报2025，Vol.32Issue(1)：65-76,12.DOI:10.16488/j.cnki.1005-9873.2025.01.008
促天麻种子萌发的广义小菇属真菌物种多样性
Preliminary Study on Species Diversity of Symbiotic Mycena sensu lato Promoting Gastrodia elata Seed Germination
摘要
Abstract
In order to understand the diversity of symbiotic fungal species promoting G.elata seed germination and explore superior symbiotic G.elata seed germination strains,extensive investigation and collection of Mycena sensu lato specimens were carried out in subtropical areas of China.A total of 324 specimens were collected,from which 401 strains were isolated.Based on morphological characteristics and initial phylogenetic analysis of ITS and LSU sequences,22 potentially functional strains were selected for co-germination experiments with G.elata seeds.The results showed that 18 of the 22 strains promoted G.elata seed germination.Through further morphology observation and phylogenetic analysis,they belonged to five genera and 13 species,including one species of Atheniella,one species of Favolaschia,eight species of Mycena,one species of Panellus,and two species of Roridomyces.Among them,13 strains further promoted G.elata seeds to develop protocorms with a pointed vegetative apex enabling further differentiation,and four strains resulted in＞90％germination rates in G.elata seeds.In particular,two M.abramsii strains and one M.maculata strain brought about a higher G.elata seed germination rate than the three prevalent commercial symbiotic M.citrinomarginata strains(SHXG,MFJ and TMMFJ)currently used for G.elata cultivation.关键词
担子菌/广义小菇属真菌/天麻种子萌发/系统发育分析Key words
Basidiomycete/Mvcena sensu lato/Gastrodia elata seed germination/phylogenetic analysis引用本文复制引用
刘丽娜,申爱荣,沈宝明,谭云,李赛男,谭著明..促天麻种子萌发的广义小菇属真菌物种多样性[J].食用菌学报,2025,32(1):65-76,12.基金项目
湖南省自然科学基金-区域联合基金(2023JJ50073) （2023JJ50073）
湖南省级林业生态保护修复及发展专项资金(SD2024A008) （SD2024A008）
