茶叶科学2025，Vol.45Issue(1)：29-42,14.
茶树DREB基因的生信分析及其调控CsPOD3的抗旱机制
Bioinformatic Analysis of DREB Genes and Regulation of CsPOD3 on Drought Tolerance Mechanisms in Camellia sinensis
摘要
Abstract
Based on the previous transcriptome data of the research group,three genes,CsDREB11,CsDREB15,and CsDREB25,were screened.Verified by RT-qPCR,their expressions were induced by drought.The phylogenetic tree,physical and chemical properties,hydrophilicity/hydrophobicity,secondary and tertiary structures,and subcellular localization were predicted and analyzed.The results show that CsDREB11,CsDREB15,and CsDREB25 are most closely related to their homolog genes in Camellia lanceoleosa.They are all unstable hydrophilic proteins.Random coils and α-helices dominate the secondary/tertiary structures.Their subcellular localizations are located in the nucleus.The preliminary study of the subject found that both CsDREB25 and CsPOD3 were highly expressed and significantly correlated under drought conditions,suggesting that there was an interaction between them.Through Y1H,DLA,and LCI experiments,it was verified that CsDREB25 can positively regulate the expression of CsPOD3,increase the activity of POD,remove excessive reactive oxygen species,and improve the drought tolerance of tea plants.关键词
茶树/干旱胁迫/DREBs/过氧化物酶/生物信息学分析Key words
Camellia sinensis/drought stress/DREBs/POD/bioinformatic analysis分类
农业科学引用本文复制引用
徐容,邓智英,邵陈禹,罗玉麒,仇舒琪,王聪,周玲红,刘仲华,沈程文..茶树DREB基因的生信分析及其调控CsPOD3的抗旱机制[J].茶叶科学,2025,45(1):29-42,14.基金项目
国家自然科学基金(32372765)、国家重点研发计划(2022YFD1600801)、湖南省现代农业产业技术体系(HARS-10)、湖南省自然科学区域联合基金(2023JJ50061) （32372765）
