茶叶科学2025，Vol.45Issue(1)：110-120,11.
茶园根际解磷菌的筛选及其对茶叶产量、品质及土壤性质的影响
Screening of Phosphate-solubilizing Bacteria in the Rhizosphere of Tea Gardens and Their Effects on Tea Yield,Quality and Soil Properties
摘要
Abstract
The purpose of this study was to screen phosphate-solubilizing bacteria with the ability to produce indoleacetic acid(IAA)from the rhizosphere of tea gardens.The rhizosphere soil was collected from Yingshan Couty and Enshi Tujia and Miao Autonomous Prefecture tea gardens in Hubei Province,and the phosphate-solubilizing bacteria were screened by plate transparent circle method.The phosphate-solubilizing and IAA-producing abilities were used as indicators for re-screening.The strains were identified by morphological characteristics and 16 S rDNA sequence analysis,and their growth-promoting characteristics were studied.The effects of strains on tea yield,quality and soil properties were studied by field experiments.The results show that the phosphate-solubilizing bacterium DFP-24 screened from the rhizosphere soil of tea plants had good IAA-producing ability and was identified as Burkholderia arboris.At the same time,the strain had the growth-promoting characteristics such as siderophore production,1-aminocyclopropane-1-carboxylic acid(ACC)deaminase production and nitrogen fixation ability.Field experiments show that the application of DFP-24 strain could increase the density of tea buds,significantly increase the total phosphorus content of tea,increase the free amino acid content of tea,reduce the ratio of phenol to ammonia,affect the quality of tea,and improve the soil properties.In summary,the strain DFP-24 has the application potential to develop microbial fertilizers.The research results could provide strain resources and theoretical basis for the development and application of rhizosphere growth-promoting bacterial fertilizer for tea plants.关键词
解磷菌/茶叶品质/促生作用/肥效Key words
phosphate-solubilizing bacteria/tea quality/growth-promoting effect/fertilizer efficiency分类
农业科技引用本文复制引用
马雪晴,吴华伟,曹春霞,郑娇莉..茶园根际解磷菌的筛选及其对茶叶产量、品质及土壤性质的影响[J].茶叶科学,2025,45(1):110-120,11.基金项目
湖北省中央引导地方科技发展专项(2022BGE250)、湖北省重点研发计划项目(2023BBB148) （2022BGE250）
