乳业科学与技术2025，Vol.48Issue(1)：14-19,6.DOI:10.7506/rykxyjs1671-5187-20241011-094
牛乳中β-乳球蛋白的中红外快速检测方法建立
Establishment of a Rapid Detection Method for β-Lactoglobulin in Milk by Mid-infrared Spectroscopy
黄建辉 1张彦辉 2张耀广 2赵翠琴2
作者信息
- 1. 河北省食品检验研究院,河北 石家庄 050021
- 2. 君乐宝乳业集团股份有限公司,农业农村部乳品质量安全控制重点实验室,河北 石家庄 050021
- 折叠
摘要
Abstract
In this study,a prediction model based on Fourier transform mid-infrared spectroscopy was developed for the rapid detection of β-lactoglobulin in milk.The content of β-lactoglobulin in 260 different batches of milk samples was determined as reference by high performance liquid chromatography(HPLC),and the mid-infrared spectra of raw milk were collected using an Fourier transform mid-infrared spectrometer.The effective wavebands were selected,and the background noise was eliminated by Savitsky-Golay(SG)smoothing,first-order derivative or second-order derivative pretreatment before the establishment of the β-lactoglobulin prediction model by using partial least squares regression(PLSR).The results showed that SG five-point smoothing second-order derivative was the optimum preprocessing method.The PLSR model exhibited good prediction accuracy with correlation coefficient(R2)of 0.932 for the calibration set,root mean square error of calibration(RMSEC)of 0.049%,R2 of 0.923 for the calibration set,and root mean square error of prediction(RMSEP)of 0.057%.关键词
傅里叶变换中红外光谱技术/β-乳球蛋白/牛乳/偏最小二乘回归法Key words
Fourier transform mid-infrared spectroscopy/β-lactoglobulin/milk/partial least squares regression分类
轻工纺织引用本文复制引用
黄建辉,张彦辉,张耀广,赵翠琴..牛乳中β-乳球蛋白的中红外快速检测方法建立[J].乳业科学与技术,2025,48(1):14-19,6.
