食品工业科技2025，Vol.46Issue(6)：179-186,8.DOI:10.13386/j.issn1002-0306.2024030409
柠檬草精油的抑菌稳定性和抗氧化活性
Antibacterial Stability and Antioxidant Activity of Essential Oils from Cymbopogon citratus
摘要
Abstract
Objective:To study the chemical components and antioxidant activity of Cymbopogon citratus essential oil and investigate the effect of processing and storage conditions on its antibacterial stability.Methods:The essential oil was extracted by steam distillation,and its chemical components were analyzed by GC-MS.The effects of temperature,ultraviolet rays,pH,and CaCl2 on the antibacterial activity were determined by filter-paper method and the antioxidant activity was determined by the ABTS+·,DPPH and O2·radical-scavenging assay and the reducing capacity for Fe3+.Results:A total of 49 compounds were identified,and 12 compounds with contents greater than 1％,accounting for 88.11％,with citral(26.73％),neral(18.57％),and β-myrcene(9.30％)as the main components.The essential oil of Cymbopogon citratus showed strong antibacterial activity.The inhibitory effect against Staphylococcus aureus(25.43±0.24 mm)was better than that of Escherichia coli(23.54±0.67 mm)and Bacillus subtilis(18.33±0.03 mm)(P＜0.05).The antibacterial activity of the essential oil were stable under ultraviolet rays,but were unstable under different temperature and pH.The antibacterial activity was stronger at low temperatures and acidic conditions,and CaCl2 could increases the antibacterial effect.The essential oil showed good antioxidant activity,the scavenging ability of ABTS+·,DPPH·,O2-·and the reducing ability of Fe3+increased with the concentration of essential oil(P＜0.05),the IC50 of ABTS+·,DPPH·,O2-·were 4.36,7.09,3.13 mg/mL,respectively,which were 6.92,10.58 and 5.05 times of ascorbic acid.Conclusion:The essential oil of Cymbopogon citratus has good antibacterial activity and antioxidant activity,high temperatures and alkaline conditions should be avoided during storage and processing.关键词
柠檬草精油/化学成分/抑菌活性/稳定性/抗氧化活性Key words
essential oil of Cvmbopogon citratus/chemical component/antibacterial activity/stability/antioxidant activity分类
轻工纺织引用本文复制引用
杨丽华,徐海涵,李德艳,陈文华..柠檬草精油的抑菌稳定性和抗氧化活性[J].食品工业科技,2025,46(6):179-186,8.基金项目
怒江下游山地农业生态系统云南省野外科学观测研究站项目(202305AM340031) （202305AM340031）
2022年云南省课程思政教改项目"地方高校《植物生物学》课程思政教育的探索与实践". （）
