食品工业科技2025，Vol.46Issue(6)：187-194,8.DOI:10.13386/j.issn1002-0306.2024030449
灵芝菌丝体固态发酵青稞工艺优化及抗氧化、降血糖活性分析
Optimization of the Solid Fermentation Process of Hordeum vulgare Linn.by Ganoderma lucidum Mycelium and Its Antioxidant and Hypoglycemic Activities
摘要
Abstract
This study focuses on the optimization of the solid fermentation conditions of highland barley(Hordeum vulgare Linn.)by Ganoderma lucidum mycelium to enhance its health benefits.The optimization was guided by triterpene content,using single-factor experiments and response surface methodology.Additionally,the antioxidant and hypoglycemic activities of the fermented product were investigated.The optimal fermentation conditions(material of 38 g/250 mL,13％inoculum size,and a temperature of 29 ℃)resulted in a triterpene content of 14.83 mg/g,representing a 129.92％increase compared to the unfermented product.The antioxidant activity of highland barley significantly improved post-fermentation,as evidenced by enhanced DPPH radical,hydroxyl radical,and superoxide anion radical scavenging activities(P＜0.05).The IC50 values for DPPH,hydroxyl,and superoxide anion radicals were 1.68,4.37,and 8.18 mg/mL,respectively,indicating the order of radical scavenging rates within the concentration range of 1～5 mg/mL as DPPH radicals＞hydroxyl radicals＞superoxide anion radicals.Furthermore,the fermented product exhibited significantly increased inhibitory effects onα-amylase and α-glucosidase(P＜0.05),and the inhibition increased with the increase of concentration within the concentration range of 15～35 mg/mL.The findings suggested that the Ganoderma lucidum-highland barley product holds potential as a functional food with substantial nutritional and health benefits.关键词
青稞/灵芝/固态发酵/抗氧化/降血糖Key words
Hordeum vulgare Linn./Ganoderma lucidum/solid fermentation/antioxidant activity/hypoglycemic activity分类
生物科学引用本文复制引用
孟缘,关颖贤,方潇,吴曼,高仙仙,张东晗,江洁..灵芝菌丝体固态发酵青稞工艺优化及抗氧化、降血糖活性分析[J].食品工业科技,2025,46(6):187-194,8.基金项目
大连民族大学大学生创新创业训练计划项目(202312026108) （202312026108）
大连民族大学"太阳鸟"学生科研项目(202319) （202319）
大连民族大学·铜仁科学院联合科研项目(2019110057) （2019110057）
大连民族大学服务国家战略专项项目(2020fwgj020) （2020fwgj020）
中央高校基本科研业务费资助(0919-140279). （0919-140279）
