食品工业科技2025，Vol.46Issue(6)：195-205,11.DOI:10.13386/j.issn1002-0306.2024040240
抗沙门氏菌后生元的筛选及其原始菌株全基因组分析
Screening of Postbiotics against Salmonella and Whole Genome Analysis of the Original Strain
黄燕 1苏悦 1李施扬 2祁雪鹤 1贾嫒 1姜毓君 1杨鑫焱 1满朝新1
作者信息
- 1. 东北农业大学食品学院,黑龙江哈尔滨 150030
- 2. 黑龙江省质量监督检测研究院,黑龙江哈尔滨 150030
- 折叠
摘要
Abstract
Objective:To screen postbiotics with exceptional anti-Salmonella activity and develop postbiotic raw materials with industrialization value.Methods:The anti-Salmonella potential of 30 Lactobacillus postbiotic strains was evaluated,focusing on their ability to inhibit the growth of Salmonella enterica serovar Typhimurium(ST).The top five most effective strains were identified,and further assays were conducted to assess their capacity to inhibit ST adhesion and invasion in HT-29 cells.The most significant effective postbiotic was selected for whole genome sequencing and safety evaluation of its originating strain.Results:Lactobacillus plantarum(L.plantarum)JM015 postbiotic exhibited a notable inhibition zone diameter of 15.12±0.53 mm.It effectively reduced the adhesion and invasion of Salmonella to HT-29 cells by more than 90％.Whole genome sequencing of L.plantarum JM015 revealed four bacteriostasis-related gene clusters related to repression:cyclic-lactone-autoinducer,terpene,ribosomally synthesized and post-translationally modified peptide(RiPP)-like,and type Ⅲ polyketide synthases(PKS).Additionally,it harbors a gene cluster associated with plantaricin production and several genes linked to adhesion,such as fibronectin,glyceraldehyde 3-phosphate dehydrogenase(GAPDH),and triosephosphate isomerase.The safety of the original strain was confirmed through tests for indole production,hemolysis,and nitrate reductase activity.Conclusion:The L.plantarum JM015 postbiotic demonstrates potent anti-Salmonella activity and can as a novel postbiotic ingredient for product development.关键词
乳酸菌/后生元/鼠伤寒沙门氏菌/抗菌/全基因组/安全性评价Key words
Lactobacillus/postbiotic/Salmonella enterica serovar Typhimurium/antibacterial/whole-genome/safety evaluation分类
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黄燕,苏悦,李施扬,祁雪鹤,贾嫒,姜毓君,杨鑫焱,满朝新..抗沙门氏菌后生元的筛选及其原始菌株全基因组分析[J].食品工业科技,2025,46(6):195-205,11.
