食品工业科技2025，Vol.46Issue(6)：231-241,11.DOI:10.13386/j.issn1002-0306.2024030456
纳溪特早茶'乌牛早'茶多糖提取纯化及免疫活性研究
Extraction,Purification and Immune Activity of Polysaccharide from Naxi Wuniu Tea
摘要
Abstract
Objective:The aim of this study was to isolate and purify the polysaccharide from Naxi Wuniu special morning tea,and the antioxidant and immune activities were further investigated.Methods:Firstly,the tea polysaccharides were extracted by hot water extraction method,and further purified by DEAE-52 and dextranan gel G200.Then,the basic physicochemical properties of polysaccharide were studied.And the antioxidant activities were evaluated by determined the 1,1 Diphenyl-2-trinitrobenzene hydrazine(DPPH)free radical scavenging capacity,2,2'-diazo-di-3-ethylbenzothiazolin-6-sulfonic acid(ABTS)cationic free radical scavenging capacity,and oxygen radical absorbance capacity(ORAC).Then,the safeties of tea polysaccharide were analyzed,including endotoxin detection,RAW264.7 cells and human embryonic kidney cell 293(HEK293)toxicity detection.Finally,the effects of tea polysaccharide on RAW264.7 cytokines(interleukin-6,IL6,tumor necrosis factor α,TNF-α,NO)release and the corresponding mRNA secretion levels,in order to obtain the immune-enhancing activity of tea polysaccharide.Results:A purified polysaccharide fraction WNp was obtained from Naxi Wuniu morning tea by using DEAE-52 anion exchange resin and Sephadex G-200.Physicochemical analysis showed that the purity of WNp was 90.26％,the protein content of WNp was 1.37％,and WNp did not contain triple helix structure.WNp was composed of glucose(Glc),galactose(Gal),arabinose(Ara),mannose(Man),rhamnose(Rha),glucuronic acid(GlcA)and galacturonic acid(GalA)at the mole ratio of 0.4∶32.6∶2.3∶4.7∶8.7∶0.3∶40.3,respectively.The molecular weight of WNp was 82.61 kDa.WNp showed significant scavenging abilities on DPPH and ABTS+free radicals and a high ORAC value,that was,it had good antioxidant activities.Finally,WNp could up-regulate the mRNA expression of cytokines(IL-6,TNF-α)and NO,thereby promoting cytokine secretion(IL-6,TNF-α)and NO release,and then activate the initial immune response of RAW264.7 cells,which achieved the immuno-enhancing activity.Conclusion:The research results provide scientific basis for the deep processing and utilization of Naxi Wulu tea source.关键词
纳西乌牛特早茶/多糖/结构/抗氧化性/免疫活性Key words
Naxi Wuniu special morning tea/polysaccharides/structure/antioxidant activity/immuno-enhancing activity分类
轻工纺织引用本文复制引用
赖玲,杨潇,尹琳,陈小雨,阳辉蓉,彭取敏,刘军,曾英杰..纳溪特早茶'乌牛早'茶多糖提取纯化及免疫活性研究[J].食品工业科技,2025,46(6):231-241,11.基金项目
西南民族大学研究生创新型科研项目(ZD2023050) （ZD2023050）
四川省自然科学基金项目(2023NSFSC1200) （2023NSFSC1200）
四川省天府峨眉计划青年科技人才项目(川峨眉第2260号) （川峨眉第2260号）
攀西特色作物研究与利用四川省重点实验室资助课题(ZYN2023037). （ZYN2023037）
