食品工业科技2025，Vol.46Issue(6)：282-292,11.DOI:10.13386/j.issn1002-0306.2024040251
GC-MS结合化学计量学表征腐生葡萄球菌MF B8对臭鳜鱼挥发性风味的影响
GC-MS Combined with Chemometrics for Characterizing the Effect of Staphylococcus saprophytic MF B8 on Volatile Flavor Compounds in Stinky Mandarin Fish
摘要
Abstract
To investigate the effect of Staphylococcus saprophyticus MF B8 from stinky mandarin fish on the volatile flavor of stinky mandarin fish,four experimental groups were set up using mandarin fish as raw material:fresh mandarin fish(S1),uninoculated fermentation(S2),inoculated fermentation by adding 1％MF B8(S3)and 2％MF B8(S4).The volatile flavor components were analyzed using headspace solid phase microextraction gas chromatography mass spectrometry(HS-SPME-GC-MS).And combined with chemometrics,identify the characteristic"stink"substances of stinky mandarin fish.The results showed that a total of 87 volatile compounds were identified by GC-MS,including 22 alcohols,11 aldehydes,8 ketones,9 acids,8 esters,23 hydrocarbons,2 sulfur-containing compounds,2 nitrogen-containing compounds,and 2 other compounds.Compared with the fresh mandarin fish,the amount of aldehydes and hydrocarbons in fermented samples decreased,while the amount of acids,alcohols,esters,ketones,and nitrogen-containing compounds increased.Through orthogonal partial least squares discriminant analysis(OPLS-DA),variable importance in projection(VIP),and odour activity value(OAV),indicated that indole,trimethylamine,acetic acid and dimethyl disulphide,may be the key characteristic"stink"substances in fermented stinky mandarin fish inoculated by Staphylococcus saprophyticus MF B8.The research results provide a theoretical basis for the formation of volatile flavor compounds in fermented fish products.关键词
腐生葡萄球菌/臭鳜鱼/挥发性风味物质/GC-MS/化学计量学Key words
Staphylococcus saprophyticus/stinky mandarin fish/volatile flavor compounds/GC-MS/chemometrics分类
轻工纺织引用本文复制引用
罗格格,杨明柳,王浩,谢宁宁,郑海波,周迎芹..GC-MS结合化学计量学表征腐生葡萄球菌MF B8对臭鳜鱼挥发性风味的影响[J].食品工业科技,2025,46(6):282-292,11.基金项目
安徽科技学院人才项目(SPWD202001) （SPWD202001）
安徽省教育厅科研项目(KJ2020A0069) （KJ2020A0069）
安徽省省级科技特派团项目(2023tpt141) （2023tpt141）
安徽省农业科学院科研平台项目(2023YL010,2023YL017) （2023YL010,2023YL017）
十四五"国家重点研发计划重点专项(2022YFD2100902) （2022YFD2100902）
安徽省重点研究与开发计划项目(202004a06020033). （202004a06020033）
