食品科学2025，Vol.46Issue(6)：38-46,9.DOI:10.7506/spkx1002-6630-20240404-038
发酵核桃粕中有助于维持血糖健康水平肽的分析及降血糖机制
Analysis of Peptides in Fermented Walnut Meal Contributing to Maintain Healthy Blood Glucose Levels and Underlying Hypoglycemic Mechanism
摘要
Abstract
This study was undertaken in order to select peptides with blood glucose regulatory activity from fermented walnut meal with fungi.Animal feeding trials were conducted for overall evaluation of the crude peptide extract,which was subsequently fractionated by Sephadex G-50 column chromatography and high performance liquid chromatography(HPLC).The resulting fractions were tested for inhibitory activity against α-amylase,α-glucosidase and dipeptidase-IV.Liquid chromatography-tandem mass spectrometry(LC-MS/MS)and bioinformatics were combined to determine shared and differential peptides among fractions IV,V and VI and obtain the major active peptides,which were then quantified by LC.The proteins from which the active peptides were derived were identified by searching the UniProt online platform,and their interactions with the above three enzymes were analyzed by molecular docking.The results showed that the crude peptides effectively controlled body mass,food intake and blood glucose,mitigated insulin resistance and improved glucose tolerance in type II diabetic mice.Fractions IV,V and VI had low molecular mass,which were separated by Sephadex G-50 column chromatography.Fraction V showed no significant difference in inhibitory activity against α-amylase and α-glucosidase but exhibited a significant difference in dipeptidase-IV inhibitory activity compared with fractions IV and VI.Comparative analysis showed that peptides MMRPDEDEQEGQRQ and LPLLR,which were found both in both V and VI,accounted for 82.3%of the total peptide content,and the peptides contributed to 94.3%of the inhibitory activity against DPP-IV.Therefore,these two peptides are very promising for glycaemic regulation.关键词
核桃肽/降血糖/2型糖尿病小鼠/多肽序列/分子对接Key words
walnut peptides/hypoglycemia/type 2 diabetic mice/peptide sequences/molecular docking分类
轻工业引用本文复制引用
刘莹莹,庞晓宇,郭聪聪,檀建新,田益玲..发酵核桃粕中有助于维持血糖健康水平肽的分析及降血糖机制[J].食品科学,2025,46(6):38-46,9.基金项目
"十四五"国家重点研发计划重点专项(2022YFD1600402) （2022YFD1600402）
河北省特色油料农业产业技术体系配套农机与产品加工专项(HBCT2024050206) （HBCT2024050206）
河北省湿地保护与绿色发展协同创新中心项目(2023hbxczx1-11) （2023hbxczx1-11）
