食品科学2025，Vol.46Issue(6)：73-79,7.DOI:10.7506/spkx1002-6630-20241016-095
不同接枝顺序三元共价复合物对蓝莓花青素的稳态作用
Stabilization Effect of Ternary Covalent Complexes with Different Grafting Sequences on Blueberry Anthocyanins
摘要
Abstract
In this study,covalent complexes(WPI-GG-TP and WPI-TP-GG)of whey protein isolate(WPI),gellan gum(GG)and tea polyphenol(TP)with different grafting sequences were synthesized by Maillard grafting and alkali treatment.Fourier transform infrared(FTIR)spectroscopy,fluorescence spectroscopy,and ultraviolet(UV)absorption spectroscopy confirmed the formation of covalent bonds within the ternary complexes.The stabilization effects of the ternary covalent complexes on blueberry anthocyanins(ACNs)were investigated.The WPI-GG-TP complex showed a loading capacity of 21.80%for ACNs,forming nanoparticles with an average particle size of 806.68 nm,while WPI-TP-GG exhibited a loading capacity of 18.68%,forming nanoparticles with an average particle size of 688.08 nm.Thermal analysis showed that the thermal peak temperature of ACNs increased by 2.51℃after loading onto WPI-TP-GG,indicating improved thermal stability of ACNs.The results of antioxidant and stability tests revealed that the WPI-GG-TP complex had higher scavenging capacity against 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical and storage stability after loading with ACNs,while the WPI-TP-GG complex exhibited higher hydroxyl radical scavenging activity,iron reducing power and thermal stability.Specifically,compared with free ACNs,the DPPH radical scavenging activity of WPI-GG-TP-ACNs increased by 29.62%,while the hydroxyl radical scavenging activity and iron reducing power of WPI-TP-GG-ACNs increased by 55.96%and 2.4 times,respectively.These findings provide valuable insights for the development of food delivery systems that enhance functional ingredient stability in the human digestive system.关键词
乳清蛋白/结冷胶/茶多酚/蓝莓花青素/共价复合物/纳米颗粒Key words
whey protein isolate/gellan gum/tea polyphenol/blueberry anthocyanins/covalent complexes/nanoparticles分类
轻工业引用本文复制引用
周欣,黄金路,陈梦琳,冯论益,朱桂兰..不同接枝顺序三元共价复合物对蓝莓花青素的稳态作用[J].食品科学,2025,46(6):73-79,7.基金项目
安徽省高校自然科学基金研究项目(2022AH040288 （）
2022AH052147) （）
安徽省教学研究重点项目(2022jyxm1301) （2022jyxm1301）
安徽省高校协同创新项目(GXXT-2023-041 （）
GXXT-2022-078) （）
安徽省质量工程项目(2023cxtd074) （2023cxtd074）
