中外葡萄与葡萄酒Issue(2)：12-21,10.DOI:10.13414/j.cnki.zwpp.2025.02.002
VyDHN1转化'西拉'愈伤组织遗传体系的建立及其抗逆性鉴定
Establishment of the Genetic Transformation System Overexpressing VyDHN1 Gene into Callus from Vitis vinifera L.'Syrah'and Measurement of the Resistance to Abiotic Stresses
摘要
Abstract
In this study,the Agrobacterium-mediated transgenic method into the callus of Vitis vinifera L.'Syrah'was established.By optimizing the three basic conditions of transgenic process,namely the bacterial solution concentration,infection duration and delayed culture duration,it was shown that the transformation efficiency of'Syrah'callus was the highest when the concentration of Agrobacterium solution was OD600=0.2,infection duration was 10 min and delayed culture was 21 d.The'Syrah'callus overexpressing VyDHN1 fused with GFP was obtained in this study.It was displayed that transgenic lines grew faster than wild-type callus under control condition,however the callus growth was inhibited by low temperature,drought and salt stress.The relative permeability of cell membrane,malondialdehyde content and free proline content of transgenic callus under low temperature,drought and salt stress were lower than those of WT,suggesting that overexpression of VyDHN1 could accelerate the growth rate of callus of'Syrah',and could protect the cell membrane structure to improve plant resistance to these abiotic stress.关键词
西拉/愈伤组织/遗传转化/脱水素基因/非生物胁迫抗逆性Key words
Syrah/callus/genetic transformation/dehydrin gene/abiotic stress resistance分类
园艺学与植物营养学引用本文复制引用
常贺强,刘牧青,韩国民,代玲敏..VyDHN1转化'西拉'愈伤组织遗传体系的建立及其抗逆性鉴定[J].中外葡萄与葡萄酒,2025,(2):12-21,10.基金项目
国家自然科学基金(31901982) （31901982）
齐鲁工业大学(山东省科学院)人才科研项目(2023RCKY227) （山东省科学院）
