中外葡萄与葡萄酒Issue(2)：53-61,9.DOI:10.13414/j.cnki.zwpp.2025.02.007
葡萄枝条降解菌的筛选及复合菌液降解效果研究
Screening of Grape Branch Degrading Bacteria and the Degradation Effect of Composite Bacterial Solution
摘要
Abstract
Branches fermented and returned to the field is an effective way to reuse resources.In this study,cellulose-degrading bacteria II6(Delftia tsuruhatensis)and lignin-degrading strains 11B(Bacillus velezensis)and 12YB(Pseudomonas guariconensis)were screened from grapevine branch compost samples.Their enzyme production capacities were determined,and the composite bacterial solution was further constructed to carry out grape branch composting test.The results showed that the cellulase activity concentration(CA)of strain II6 was 8.18 U·mL-1 and the filter paper enzyme activity concentration(FPA)was 8.84 U·mL-1.The two lignin-degrading bacterial strains 11B and 12YB had laccase(Lac)producing capacities of 35.35 U·mL-1 and 40.98 U·mL-1,peroxidase(Lip)producing capacities of 86.11 U·mL-1 and 116.25 U·mL-1,and manganese peroxidase(Mnp)producing capacities of 88.07 U·mL-1 and 93.93 U·mL-1.The composite solution inoculated with the three strains of bacteria was able to prolong the high temperature period of the compost and increase the branch degradation rate.In this paper,we screened out the grapevine branch decomposing bacterial strains and clarified their effects on the composting process of grapevine branches,which is of certain significance to the resource utilization of waste branches in the actual production of orchards.关键词
葡萄枝条/木质素降解菌/纤维素降解菌/枝条腐解Key words
grape branch/lignin-degrading bacteria/cellulose degrading bacteria/branch decomposition分类
园艺学与植物营养学引用本文复制引用
刘超,姜雨晴,武梦真,冯长长,杨恩帅,高振,杜远鹏..葡萄枝条降解菌的筛选及复合菌液降解效果研究[J].中外葡萄与葡萄酒,2025,(2):53-61,9.基金项目
山东省重点研发计划资助(2022TZXD0010)财政部和农业农村部:国家现代农业产业技术体系(CARS-29-zp-1) （2022TZXD0010）
