中外葡萄与葡萄酒Issue(2)：84-89,6.DOI:10.13414/j.cnki.zwpp.2025.02.010
酿酒葡萄皮渣提取物对癌细胞的抑制研究
Study on Inhibitory Effect of Wine Grape Peel Residue Extract on Cancer Cells
摘要
Abstract
To study the inhibitory effect of wine grape peel extract on human cancer cells,wine grape peel residue was extracted using methanol as solvent.MTT colorimetric analysis,DAPI staining and Annexin V-FITC/PI double staining were used to analyze its effects on cancer cell survival rate,cell morphology,cell cycle and apoptosis.The results showed that grape peel extract had different effects on different cancer cells,with the most significant inhibitory effect on A549 cells.Further study of A549 cells found,10、15 μg·mL-1 of the extract concentration resulted in a significant decrease in the number of A549 cells and nuclear shrinkage;The S phase of A549 cells was shortened,while the G1 and G2 phase were lengthened.Comprehensive analysis showed that grape peel extract caused abnormal morphology of A549 cells,which may be one of the mechanisms of inhibiting A549 cells.关键词
酿酒葡萄/皮渣提取物/癌细胞/A549细胞Key words
wine grape/peel residue extract/cancer cells/A549 cells分类
轻工业引用本文复制引用
徐明磊,孙亚楠,陈双臣..酿酒葡萄皮渣提取物对癌细胞的抑制研究[J].中外葡萄与葡萄酒,2025,(2):84-89,6.基金项目
国家自然科学基金项目(31471867) （31471867）
河南省高等学校重点科研项目(23B210008) （23B210008）
