食品工业科技2025，Vol.46Issue(7)：32-41,10.DOI:10.13386/j.issn1002-0306.2024080277
代谢组学分析揭示成熟前后小粒咖啡果皮黄酮和类胡萝卜素差异
Metabolomic Analysis Reveals Differences in Flavonoids and Carotenoids in the Peel of Coffea arabica L.before and after Ripeness
摘要
Abstract
This study examined the changes in flavonoid and carotenoid metabolites in the peel of Coffea arabica L.in mature and immature stages and their roles in coloration.Ultra-performance liquid chromatography coupled with tandem mass spectrometry(UPLC-MS/MS)was employed to analyze the metabolite profiles,in combination with principal component analysis(PCA),orthogonal partial least squares discriminant analysis(OPLS-DA),and clustering analysis.A total of 234 flavonoid metabolites and 40 carotenoid metabolites were detected,with flavonols(81 species)and lutein(34 species)being the most abundant.The total carotenoid content in the peel decreased significantly after ripening,while the total flavonoid content remained relatively stable,although anthocyanin accumulation showed the opposite pattern.Lutein and β-carotene accumulation in unripe peels were linked to green coloration,while cyanidin derivatives contributed to red coloration in ripe peels.Furthermore,unique accumulations of phytoene and phytofluene were observed in the ripe peels.This study elucidates the relationship between flavonoid and carotenoid accumulation and peel coloration during the ripening of Coffea arabica L.and provides new insights into the mechanisms underlying coffee peel coloration,offering a theoretical foundation for the utilization of coffee by-products and the development of functional foods.关键词
小粒种咖啡/咖啡果皮代谢组学/代谢物积累/黄酮类化合物/类胡萝卜素积累Key words
Coffea arabica L./coffea peels metabolomics/metabolite accumulation/flavonoids/carotenoid accumulation分类
农业科学引用本文复制引用
王祖权,谭玉龙,郭银楠,谢纯,李学俊,杜华波,俞思莹,张传利..代谢组学分析揭示成熟前后小粒咖啡果皮黄酮和类胡萝卜素差异[J].食品工业科技,2025,46(7):32-41,10.基金项目
云南省科技厅农业联合专项(202101BD070001-072) （202101BD070001-072）
云南省科技厅重大科技专项(202102AA100010-003) （202102AA100010-003）
云南省科技厅重大科技专项(202102AA310045-003) （202102AA310045-003）
云南省专家工作站(202305AF150125) （202305AF150125）
2024年大学生创新创业训练计划省级重点领域支持项目(S202410676006). （S202410676006）
