食品工业科技2025，Vol.46Issue(7)：325-336,12.DOI:10.13386/j.issn1002-0306.2024030152
热灭活芽孢杆菌DU-106对高脂秀丽隐杆线虫降脂作用
Lipid-lowering Effect of Thermally Inactivated Bacillus sp.DU-106 on High-fat C.elegans
摘要
Abstract
This study explored the lipid-lowering efficacy of thermally inactivated Bacillus sp.DU-106(TIDU-106)on Caenorhabditis elegans.A high-fat model of C.elegans was developed using a high-glucose induction method with a concentration of 10 mmol/L.To evaluate the effects of TIDU-106 on high-fat Caenorhabditis elegans,concentrations of 2.5,5.0,and 10 mg/mL were applied,assessing their impact on lifespan,locomotion,egg-laying capability,levels of reactive oxygen species(ROS),resistance to stress,activity of antioxidant enzymes,expression of genes related to lipid metabolism,and fat accumulation.Initial investigations in wild-type worms led to the selection of 5.0 mg/mL and 10 mg/mL concentrations of TIDU-106 for further examination of its lipid-lowering properties in gene-deficient worms.The results demonstrated that TIDU-106 treatment significantly increased the maximum lifespan of high-fat worms by 35.11％,43.84％,and 45.63％with varying doses(2.5,5.0,10 mg/mL)(P＜0.05).These enhancements were accompanied by improved locomotion,a higher egg-laying capacity,and a decreased both in ROS levels and mitochondrial membrane potential.Additionally,treatment with TIDU-106 resulted in increased levels of superoxide dismutase,catalase,and glutathione peroxidase,coupled with a decrease in malondialdehyde levels.Notably,TIDU-106 was found to modulate key signaling pathways related to lipid-lowering,including the SREBP signaling pathway,the insulin/IGF-1 signaling pathway,the nuclear receptor signaling pathway,and the AMPK signaling pathway,as well as key genes such as mdt-15,sbp-1,fat-5,fat-7,daf-2,daf-16,skn-1,sod-3,sir-2.1,aak-2,nhr-49,acs-2,and kat-1.Specifically,after the administration of TIDU-106,there was a significant reduction in triglyceride levels in fat-5,fat-5/fat-6,fat-7,and daf-16 mutant worms(P＜0.05),with daf-2,sir-2.1,and aak-2 mutants exhibiting no significant alterations(P＞0.05).Above findings revealed the potential of TIDU-106 to mitigate fat deposition,boost resistance to oxidative stress,and increase antioxidant enzyme activity,thereby emphasizing its promising lipid-lowering effects in high-fat C.elegans models.关键词
芽孢杆菌DU-106/热灭活/秀丽隐杆线虫/脂代谢/抗氧化Key words
Bacillus sp.DU-106/thermally inactivation/Caenorhabditis elegans/lipid metabolism/antioxidant分类
轻工纺织引用本文复制引用
李彦力,黎攀,杜冰,赵文俊,王梓宇,郭荣香,王琨,江冰雁,刘琳,陈颖仪,齐睿达..热灭活芽孢杆菌DU-106对高脂秀丽隐杆线虫降脂作用[J].食品工业科技,2025,46(7):325-336,12.基金项目
财政部和农业农村部:国家现代农业产业技术体系资助(CARS-21). （CARS-21）
