食品工业科技2025，Vol.46Issue(7)：337-345,9.DOI:10.13386/j.issn1002-0306.2024040157
青钱柳对2型糖尿病大鼠血糖及肠道GLP-1分泌的影响
Effect of Cyclocarya paliurus on Glucose Metabolism and Intestinal GLP-1 Secretion in Type 2 Diabetic Rats
摘要
Abstract
Objective:This study aimed to evaluate the hypoglycemic effect of Cyclocarya paliurus on type 2 diabetic rats and its impact on glucagon-like peptide-1(GLP-1)secretion,and to preliminarily explore its mechanism.Methods:Type 2 diabetes was induced in rats using a high-fat,high-sugar diet combined with streptozotocin.Rats were then treated with low-dose(400 mg/kg)and high-dose(800 mg/kg)Cyclocarya paliurus,or metformin(150 mg/kg),via gavage for 8 weeks.Weekly assessments included body weight,food intake,and fasting glucose levels.After 8 weeks,an oral glucose tolerance test was conducted.Serum levels of glycated hemoglobin,fasting insulin,GLP-1,alanine aminotransferase,and aspartate aminotransferase were measured.Liver glycogen levels and glucokinase(GK)activity were assessed,and liver pathology was examined using HE staining.Real-time PCR analyzed mRNA expression of GCG,PC1/3 in colon tissues,and GK in liver tissues,while immunohistochemistry was used to detect GLP-1 protein expression in colon tissues.Results:Cyclocarya paliurus treatment(both low-and high-doses)effectively reduced food intake and stabilized body weight in diabetic rats,positively influencing their diabetes condition compared to the model group.Glycated hemoglobin and the insulin resistance index were significantly lower(P＜0.01)in both low-and high-doses Cyclocarya paliurus-treated rats.Fasting insulin was significantly lower(P＜0.01),while the insulin sensitivity index was significantly higher in the high-dose group than that in the model group(P＜0.05).HE staining revealed improved liver pathology in Cyclocarya paliurus-treated groups.Immunohistochemistry and Real-time PCR results indicated increased GLP-1 expression and secretion in both circulating blood and colon tissues(P＜0.05,P＜0.01)and enhanced activity and mRNA expression of GK in liver(P＜0.01).Additionally,hepatic glycogen levels were elevated(P＜0.05,P＜0.01).Conclusion:Cyclocarya paliurus could effectively stabilize blood glucose levels,improve insulin resistance,and regulate GLP-1 synthesis and secretion,which has a certain protective effect on the liver.These effects are mediated through the regulation of the liver-intestinal axis.关键词
青钱柳/2型糖尿病/胰高血糖素样肽-1/葡萄糖激酶/肝肠轴Key words
Cyclocarya paliurus/type 2 diabetes/glucagon-like peptide-1/glucokinase/liver-intestinal axis分类
医药卫生引用本文复制引用
邹雅倩,陈靓,慕佳,王雅靖,陈絮蒙..青钱柳对2型糖尿病大鼠血糖及肠道GLP-1分泌的影响[J].食品工业科技,2025,46(7):337-345,9.基金项目
长沙市自然科学基金项目(kq2402185) （kq2402185）
湖南省自然科学基金项目(2024JJ8195) （2024JJ8195）
湖南省中医药管理局科研项目(B2024009) （B2024009）
省级大学生创新创业计划项目(S202410541080) （S202410541080）
湖南中医药大学大学本科生科研创业基金项目(2023BKS190) （2023BKS190）
湖南省药学重点学科资助. （）
