食品工业科技2025，Vol.46Issue(7)：346-354,9.DOI:10.13386/j.issn1002-0306.2024050308
山楂果胶寡糖对过氧化氢诱导的人胚肺成纤维细胞衰老的作用
Effect of Pectin Oligosaccharides from Hawthorn on the H2O2-induced Human Embryonic Lung Fibroblast Cells
摘要
Abstract
In this study,the anti-aging effects and mechanism of action of pectin oligosaccharides from hawthorn(HPOS)on H2O2-induced human embryonic lung fibroblast cells were evaluated using the MRC-5 cell line.The effects on cell viability,survival rate,β-galactosidase activity,mitochondrial membrane potential,the content of reactive oxygen species(ROS),antioxidant capacity,and the expression levels of proteins associated with the p53/p2 1/p16 signaling pathway were evaluated.The findings illustrated that compared with the model group,under the intervention of HPOS at a concentration of 0.8 mg/mL,the cell viability,survival rate,and mitochondrial membrane potential of MRC-5 cells increased by 16.4％,17.0％,and 284.3％(P＜0.01),respectively.Furthermore,the proportion of β-galactosidase-positive cells and fluorescence intensity of ROS significantly decreased by 66.9％,77.2％(P＜0.01),respectively.The superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)contents increased by 72.0％and 50.0％,respectively.Compared to that in the model group,the malondialdehyde(MDA)content decreased by 42.6％(P＜0.01)significantly.HPOS at a concentration of 0.8 mg/mL significantly inhibited p53,p21,and p 16 expression in the p53/p21/p16 pathway(P＜0.01).Thus,HPOS ameliorates the H2O2-induced aging of MRC-5 cells by inhibiting apoptosis and oxidative stress damage along with regulating the p53/p21/p 16 pathway,thereby providing a scientific basis for the development of HPOS as a functional food ingredient that acts against aging.关键词
山楂果胶寡糖/衰老/细胞凋亡/抗氧化能力/p53/p21/p16通路Key words
pectin oligosaccharides from hawthorn/aging/cell apoptosis/antioxidant capacity/p53/p21/p 16 pathway分类
轻工业引用本文复制引用
李涛,纪文华,董红敬,王珍强,黄群,王晓..山楂果胶寡糖对过氧化氢诱导的人胚肺成纤维细胞衰老的作用[J].食品工业科技,2025,46(7):346-354,9.基金项目
"十四五"国家重点研发计划(2023YFC3503805) （2023YFC3503805）
泰山学者工程(NO.tsqnz20231230) （NO.tsqnz20231230）
济南市创新团队(202228020). （202228020）
