食品科学2025，Vol.46Issue(7)：61-68,8.DOI:10.7506/spkx1002-6630-20241114-110
共价修饰时间对乳铁蛋白-表没食子儿茶素没食子酸酯复合物结构及成骨活性的影响
Effect of Covalent Modification Duration on the Structure and Osteogenic Activity of Lactoferrin-Epigallocatechin Gallate Complexes
摘要
Abstract
To enhance the application value of lactoferrin(LF)in the health field by exploiting nutrient interactions,this study explored the covalent binding potential between LF and epigallocatechin gallate(EGCG).The aim is to augment the biological efficacy of LF while concurrently reducing its production costs.The effects of different durations of covalent reaction on the structure,thermal stability,and osteogenic activity of LF-EGCG complexes were investigated using circular dichroism(CD)spectroscopy,Fourier transform infrared(FTIR)spectroscopy,endogenous fluorescence spectroscopy,Raman spectroscopy,and differential scanning calorimetry(DSC).The results revealed that a 0.5 h covalent reaction between EGCG and LF led to significant structural changes in LF,while reducing the surface hydrophobicity to 1 304.00(85.03%lower than that of LF).Furthermore,the resulting complex at a concentration of 100 μg/mL significantly promoted osteoblast proliferation(which increased the proliferation rate to 1.43 after 24 hours)and enhanced alkaline phosphatase activity(after treatment for 7 days,the enzyme activity increased to 15.27 U/g,56.61%higher compared with the control group).This study provides new ideas for the functional modification of LF and lays the foundation for its application in the field of bone health.关键词
乳铁蛋白/表没食子儿茶素没食子酸酯/共价反应/结构表征/成骨活性Key words
lactoferrin/epigallocatechin gallate/covalent reaction/structural characterization/osteogenic activity分类
轻工业引用本文复制引用
翟珍妮,廉小妮,郑佳雨,陈慧伶,樊凤娇,方勇..共价修饰时间对乳铁蛋白-表没食子儿茶素没食子酸酯复合物结构及成骨活性的影响[J].食品科学,2025,46(7):61-68,8.基金项目
国家自然科学基金青年科学基金项目(32202032) （32202032）
国家自然科学基金面上项目(32272319) （32272319）
江苏省研究生科研与实践创新计划项目(KYCX23_1891 （）
KYCX24_1932) （）
江苏高校优势学科建设工程资助项目(PAPD) （PAPD）
