食品科学2025，Vol.46Issue(7)：92-99,8.DOI:10.7506/spkx1002-6630-20240809-081
化学降解方法对铁皮石斛多糖理化性质和抗衰老活性的影响
Effect of Chemical Degradation on Physicochemical Properties and Anti-aging Activity of Dendrobium officinale Polysaccharides
摘要
Abstract
In order to study the effect of chemical degradation on the physicochemical properties and anti-aging activity of Dendrobium officinale polysaccharide(DOP),four degraded polysaccharides were prepared by vitamin C(VC),H2O2,H2O2-VC and citric acid methods.The results showed that DOP was primarily composed of component Ⅰ(tR=4.5 min),with relative molecular mass of 1.168×107 Da.The molecular mass distribution of DOP did not change after treatment with H2O2 and citric acid,showing that neither treatment with H2O2 nor citric acid degraded DOP.VC-degraded DOP was composed of two components with relative molecular masses of 9.24×105 and 1.49×104 Da(tR=5.923 and 8.239 min),demonstrating that VC alone and combined with H2O2 could partially degrade DOP.All treatments resulted in no significant change in the content of total sugar in DOP but increased the protein content.The results of Fourier transform infrared spectroscopy(FTIR)showed that none of these four chemical degradation methods destroyed the primary functional groups of DOP.Monosaccharide composition analysis showed that DOP and VC-degraded DOP were mainly composed of mannose and glucose.The results of particle size and zeta potential measurements showed that the particle size and zeta potential absolute value decreased after the degradation of VC;the results of electron microscope scanning observations showed that the polysaccharide structure became loose after degradation.The results of in vitro anti-aging activity test showed that both DOP and VC-degraded DOP promoted collagen secretion and inhibited elastase,and the elastase inhibitory effect of VC-degraded DOP at a concentration of 200 μg/mL was more pronounced than that of DOP.In general,VC-degraded DOP showed better elastase inhibitory effect.Both DOP and VC-degraded DOP had collagenase inhibitory effects.This study provides a theoretical reference for the application of DOP in various important areas such as anti-aging foods,pharmaceuticals and health products.关键词
铁皮石斛/多糖/降解/理化性质/抗衰老Key words
Dendrobium officinale/polysaccharide/degradation/physicochemical properties/anti-aging分类
轻工业引用本文复制引用
曾燚,杨益娜,邵会艳,李严朗,席星雨,杨柳,胡江苗..化学降解方法对铁皮石斛多糖理化性质和抗衰老活性的影响[J].食品科学,2025,46(7):92-99,8.基金项目
云南省重点项目(202401AS070075) （202401AS070075）
中国科学院战略生物资源计划项目(KFJ-BRP-007-019) （KFJ-BRP-007-019）
