食品科学2025，Vol.46Issue(7)：125-134,10.DOI:10.7506/spkx1002-6630-20241105-030
1种CYTH样蛋白YjbK对食源性蜡样芽孢杆菌呕吐毒素Cereulide生物合成过程的影响
Effect of YjbK,a CYTH-like Protein,on the Biosynthesis of Cereulide in Foodborne Bacillus cereus
摘要
Abstract
Our aim was to explore the synthesis mechanism of cereulide in Bacillus cereus,causing vomiting-type food poisoning.In this study,the potential regulatory gene yjbK was knocked out by homologous recombination,revealing that yjbK positively regulated the synthesis of cereulide in B.cereus.Through bioinformatics analysis,the potential phosphohydrolase activity of YjbK and the key sites of ExExK were determined.Subsequently,point mutant expression and complementary strains of YjbK were constructed,and it was clarified that mutations at sites E6 and E8 significantly reduced the activity of phosphohydrolase and the yield of cereulide,thereby confirming that phosphohydrolase activity positively regulated the synthesis of cereulide in B.cereus.This study provides a new theoretical basis for understanding the molecular regulatory mechanism of cereulide synthesis.关键词
蜡样芽孢杆菌/呕吐毒素/YjbK蛋白/腺苷酸环化酶/磷酸水解酶Key words
Bacillus cereus/cereulide/YjbK protein/adenylate cyclase/phosphohydrolase分类
轻工业引用本文复制引用
梁欣敏,李佳珣,李阳福,秦科元,李湘,张菊梅,王涓,吴清平,丁郁..1种CYTH样蛋白YjbK对食源性蜡样芽孢杆菌呕吐毒素Cereulide生物合成过程的影响[J].食品科学,2025,46(7):125-134,10.基金项目
国家自然科学基金面上项目(32272424) （32272424）
广东省基础与应用基础研究重大项目(2020B0301030005) （2020B0301030005）
"十四五"国家重点研发计划重点专项(2022YFF1100700) （2022YFF1100700）
