食品科学2025，Vol.46Issue(7)：143-150,8.DOI:10.7506/spkx1002-6630-20241008-016
基于表面等离子体共振技术筛选马氏珠母贝肉酶解产物中血管紧张素转换酶抑制肽
Selection of Angiotensin I Converting Enzyme Inhibitory Peptides from Enzymatic Hydrolysate of Pinctada martensii Using Surface Plasmon Resonance
摘要
Abstract
Surface plasmon resonance(SPR)technology was used to analyze the binding of the three ultrafiltration fractions of the protein hydrolysate of Pinctada martensii(PHPM)with angiotensin I converting enzyme(ACE)as a protein ligand.The amino acid sequences of the bound peptides were identified by mass spectrometry(MS)and those with strong inhibitory potential against ACE were selected and synthesized.The in vitro ACE inhibitory activity and inhibition type of the peptides were studied as well as their interaction with ACE.It was found that the ultrafiltration fraction with a molecular mass of 3 000-5 000 Da had a strong binding signal to ACE.Among the four synthesized ACE inhibitory peptides,SLPWPMKPMNLIE had the lowest 50%inhibitory concentration(IC50)value and bound to the hydrophobic pocket of the C domain in ACE through hydrogen bonding.关键词
马氏珠母贝/血管紧张素转换酶抑制肽/表面等离子体共振/酶抑制动力学/分子对接Key words
Pinctada martensii/angiotensin I converting enzyme inhibitory peptide/surface plasmon resonance/enzymatic inhibition kinetics/molecular docking分类
轻工纺织引用本文复制引用
夏小雨,高加龙,温财兴,曹文红,秦小明,李钰金,林海生,陈忠琴,郑惠娜,朱国萍..基于表面等离子体共振技术筛选马氏珠母贝肉酶解产物中血管紧张素转换酶抑制肽[J].食品科学,2025,46(7):143-150,8.基金项目
贝类产业技术体系项目(CARS-49) （CARS-49）
湛江市科技计划项目(230906164548720) （230906164548720）
山东省重点扶持区域紧缺人才项目(鲁发改动能办[2024]658号) （鲁发改动能办[2024]658号）
