食品科学2025，Vol.46Issue(7)：162-171,10.DOI:10.7506/spkx1002-6630-20241016-098
青钱柳叶中黄酮类化合物提纯、鉴定以及改善胰岛素抵抗活性
Purification and Identification of Flavonoids from Cyclocarya paliurus Leaves and Ameliorative Effect on Insulin Resistance
摘要
Abstract
In order to develop natural plant-derived hypoglycemic agents for the treatment of type 2 diabetes,an extract rich in flavonoids with a brownish-yellow color was obtained from the leaves of Cyclocarya paliurus,a medicinal and edible plant,by sequential refluxing ethanol extraction and polyamide column chromatography.In the extract,17 flavonoids were identified by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-QTOF-MS).Meanwhile,the flavonoid-rich extract and the standards of its three representative flavonoid components were evaluated for their ameliorative effect on insulin resistance.A HepG2 cell model of insulin resistance was established under optimized conditions(4.5 g/L glucose,0.001 μmol/L insulin,3.5 μmol/L dexamethasone,and 24 induction),which was stable for 48 h and could be treated with metformin,an oral hypoglycemic agent.The flavonoid-rich extract as well as hyperoside and scutellarin at a concentration of 10-2 mg/mL and kaempferol at 10-4 mg/mL were the most effective in ameliorating insulin resistance.Furthermore,we found that the mechanism for the ameliorative effect of flavonoids from Cyclocarya paliurus leaves on insulin resistance may be by alleviating glycogen metabolism disorders and increasing oxidative stress in the insulin resistance model.关键词
青钱柳叶/黄酮类化合物/胰岛素抵抗/HepG2细胞Key words
Cyclocarya paliurus leaves/flavonoids/insulin resistance/HepG2 cells分类
轻工纺织引用本文复制引用
徐伟翔,张晴晴,席慧婷,刘忠伟,王远兴..青钱柳叶中黄酮类化合物提纯、鉴定以及改善胰岛素抵抗活性[J].食品科学,2025,46(7):162-171,10.基金项目
国家自然科学基金地区科学基金项目(31960090 （）
32160562) （）
南昌大学食品科学与技术国家重点实验室自由探索项目(SKLF-ZZB-202129 （）
SKLF-ZZB-202324) （）
