食品科学2025，Vol.46Issue(7)：283-291,9.DOI:10.7506/spkx1002-6630-20241011-064
采前喷施壳寡糖对采后西梅黑斑病的控制
Control of Postharvest Prune Black Spot Disease by Preharvest Chitosan Oligosaccharide Spraying
摘要
Abstract
This study investigated the effect of preharvest application of chitosan oligosaccharides(COS)on the control of postharvest black spot disease in'French'prune fruits.Prune fruits were sprayed with different concentrations of COS(0.05%,0.10%and 0.20%)or water as a control at four developmental stages(fruit set,enlargement,color change and maturity).After preharvest COS treatment,the fruits were inoculated with Alternaria alternata and stored for 90 days at(1.0±1.0)℃and a relative humidity of 90%-95%.Samples were taken every 15 days to measure relevant indicators.The in vitro inhibitory effect of COS on A.alternat was also tested.The results showed that COS significantly hindered the colony growth and spore germination of A.alternata.Scanning electron microscopic examination showed that the hyphae of A.alternata treated with COS exhibited a rough surface and were irregular in thickness with partial collapse,indicating notable disruption of the hyphal integrity.Notably,the preharvest application of 0.10%COS significantly inhibited the incidence of black spot disease and lesion progression in prune fruits inoculated with A.alternata during postharvest storage.Furthermore,preharvest COS treatment elevated the activities of superoxide dismutase(SOD),catalase(CAT),chitinase(CHT),β-1,3-glucanase(GLU),phenylalanine ammonia-lyase(PAL),4-coumarate:coenzyme A ligase(4CL),and cinnamate-4-hydroxylase(C4H),and facilitated the accumulation of total phenols,flavonoids,and lignin along with a rapid surge in H2O2 levels during the early storage phase,which was maintained at lower levels in later stages.These findings suggest that the preharvest application of COS can enhance the black spot disease resistance of postharvest prune fruits by directly exerting an antifungal effect and inducing disease resistance.This study provides a new idea and theoretical basis for disease prevention and control in prune fruits.关键词
壳寡糖/采前喷施/西梅/体外抑菌/诱导抗病性Key words
chitosan oligosaccharides/preharvest spraying/prune/in vitro fungal inhibition/induced disease resistance分类
轻工纺织引用本文复制引用
李文博,张新祺,赵亚婷,田瑞,吴颖颉,范雅青,张璇,李佳欣,朱璇..采前喷施壳寡糖对采后西梅黑斑病的控制[J].食品科学,2025,46(7):283-291,9.基金项目
自治区重点研发计划项目(2022B02018-2) （2022B02018-2）
新疆维吾尔自治区天池英才引进计划-青年博士人才项目 （）
南京农业大学-新疆农业大学联合基金项目(KYYJ201908) （KYYJ201908）
