食品与机械2025，Vol.41Issue(2)：56-64,9.DOI:10.13652/j.spjx.1003.5788.2024.81239
快速溶剂萃取—高效液相色谱—电感耦合等离子体质谱法测定富硒食用油中8种硒形态
Determination of eight selenium species in selenium-enriched edible oil with accelerated solvent extraction-high performance liquid chromatography-inductively coupled plasma mass spectrometry
摘要
Abstract
[Objective]In this study,an optimized strategy was proposed to address the limitations of current methods for detecting selenium species in selenium-enriched edible oils,such as the transformation between selenium species,long enzymatic hydrolysis time,and the limited number of detectable selenium species.[Methods]Protease K-assisted hydrolysis was performed using accelerated solvent extraction,and a high performance liquid chromatography-inductively coupled plasma mass spectrometry(HPLC-ICP-MS)method was established.[Results]At 40℃,with a proteinase K dosage of 35 mg and a static extraction time of 5 min,the extraction rate of selenium species could reach a maximum of(83.65±2.36)%.Eight target selenium species could be effectively separated within 20 min,with good linearity in the range of 2～100 μg/L(R2≥0.999 3),detection limits of 0.20～0.60 μg/kg,quantification limits of 0.60～1.80 μg/kg,spiked recovery rates between 82.3%and 112.3%,and relative standard deviations≤5.60%.[Conclusion]This method improves the efficiency and accuracy of selenium speciation analysis.关键词
硒形态/电感耦合等离子体质谱法/混合模式色谱/动态反应池/有机进样/快速溶剂Key words
selenium species/inductively coupled plasma mass spectrometry/mixed-mode chromatography/dynamic reaction cell/organic sampling/accelerated solvent引用本文复制引用
梁志森,张书迪,凌逍,陈玉珍..快速溶剂萃取—高效液相色谱—电感耦合等离子体质谱法测定富硒食用油中8种硒形态[J].食品与机械,2025,41(2):56-64,9.基金项目
福建省自然科学基金(编号:2022J05321) （编号:2022J05321）
广东省市场监督管理局科技项目(编号:2023CZ07) （编号:2023CZ07）
