食品与机械2025，Vol.41Issue(2)：65-71,7.DOI:10.13652/j.spjx.1003.5788.2024.80588
氨基酸分析仪法测定动物性水产品中8种生物胺含量
Quantification of eight biogenic amines in aquatic animal products with amino acid analyzer
摘要
Abstract
[Objective]To address the cumbersome manual pre-column derivatization steps and poor derivative stability encountered in traditional chromatography when determining biogenic amines,so as to improve the efficiency and accuracy of detection.[Methods]In this study,an amino acid analyzer method is established for the simultaneous determination of eight biogenic amines(i.e.,putrescine,histamine,cadaverine,spermidine,octopamine,spermine,tyramine,and phenylethylamine)in aquatic animal products.The aquatic animal product samples are extracted using a sulfosalicylic acid solution,which is followed by high-speed refrigerated centrifugation,elution with potassium salt buffer,separation using a potassium ionic sulfonic acid based strong acid cation exchange chromatographic column,online derivatization with ninhydrin at 130℃,and detection at 570 nm.[Results]The linear ranges for the eight biogenic amines are 1.0～50.0 mg/L with correlation coefficients(R2)greater than 0.994 7.The limits of detection range from 2.5 to 5.0 mg/kg,and the limits of quantification range from 8.3 to 16.7 mg/kg.The spike recovery rates for the three matrices range from 62.2%to 119.2%.The derivatization conditions and equipment stability are satisfactory,with relative standard deviations(RSD)of peak areas ranging from 0.43%to 3.89%and RSD of retention time ranging from 0.050%to 0.334%.[Conclusion]This developed method exhibits excellent stability,sensitivity,recovery,and accuracy.It is a rapid and simple approach for the detection of the eight biogenic amines in aquatic animal products.关键词
氨基酸分析仪/生物胺/茚三酮/在线柱后衍生/水产品Key words
amino acid analyzer/biogenic amines/ninhydrin/online post-column derivatization/aquatic products引用本文复制引用
冯洪燕,夏永生,钱亮亮,陈小娟,周静..氨基酸分析仪法测定动物性水产品中8种生物胺含量[J].食品与机械,2025,41(2):65-71,7.基金项目
江苏省市场监管局科技计划项目(编号:KJ21125123,KJ2023065) （编号:KJ21125123,KJ2023065）
