食品与机械2025，Vol.41Issue(2)：160-165,6.DOI:10.13652/j.spjx.1003.5788.2024.80444
桦木汁对斑马鱼胚胎的体外抗氧化作用及体内酶学检测
Antioxidant effects and enzymatic analysis of birch sap on zebrafish embryos
摘要
Abstract
[Objective]This study aimed to evaluate the antioxidant activity of natural birch juice from Changbai Mountain.[Methods]Antioxidant activity was measured through four chemical assays:DPPH radical scavenging rate,ABTS cation scavenging rate,hydroxyl radical scavenging rate,ferric ion reducing ability(FRAP).Additionally,antioxidant enzyme activities and oxidative stress markers were assessed using zebrafish embryo as a model organism.[Results]The antioxidant activity of birch juice increased with concentration,showing a significant positive correlation.At 1.0 mL/mL concentration,birch juice exhibited optimal effects,achieving 34.56%DPPH scavenging,18.27%ABTS scavenging,48.77%hydroxyl radical scavenging,and 90.07 μmol/L ferrous ion reduction.In zebrafish embryos,the activities of SOD,CAT,and GSH-Px were significantly elevated,along with increased MDA content,compared to the control group.[Conclusion]Natural birch juice demonstrates substantial antioxidant properties,highlighting its potential for development into natural antioxidant foods,as well as anti-aging and skin-whitening products.关键词
桦木汁/斑马鱼胚胎/抗氧化性/自由基清除率/活性测定Key words
birch juice/zebrafish embryos/antioxidant properties/free radical scavenging rate/activity determination引用本文复制引用
邢琦,陈海燕,华李羽,王超,李轲,刘纪盈..桦木汁对斑马鱼胚胎的体外抗氧化作用及体内酶学检测[J].食品与机械,2025,41(2):160-165,6.基金项目
吉林省教育厅科学技术研究项目(编号:JJKH20241707CY) （编号:JJKH20241707CY）
