食品与机械2025，Vol.41Issue(2)：173-180,8.DOI:10.13652/j.spjx.1003.5788.2024.80177
啤酒废酵母水解液养殖裂壶藻生产DHA藻油
Production of DHA algal oil from Schizochytrium sp.fed with waste beer yeast hydrolysate
摘要
Abstract
[Objective]To explore the feasibility of cultivating Schizochytrium sp.using waste beer yeast hydrolysate to produce DHA algal oil.[Methods]Ultrasound-assisted composite enzyme method was selected to break the cell wall and hydrolyze waste beer yeast,optimizing the process based on reducing sugar and total nitrogen concentrations as response variables.The hydrolysate was then used to cultivate Schizochytrium sp.to produce DHA algal oil.[Results]Under the conditions of ultrasonic power at 400 W,temperature at 60℃,pH 5.0,papain at 0.5 g,β-glucanase:papain ratio of 4:5,and hydrolysis time of 40 min,the hydrolysate achieved maximum concentrations of reducing sugar and total nitrogen,which were 20.40 g/L and 6.72 g/L,respectively.When the hydrolysate volume fraction was 75%and the inoculum volume fraction of Schizochytrium sp.was 14%,after 3 days of cultivation,the biomass,oil yield,DHA content,and DHA yield reached their maximum values of 17.14 g/L,2.88 g/L,3.13%,and 0.55 g/L,respectively,which were higher than those obtained from traditional fermentation cultures(biomass 10.29 g/L,oil yield 1.84 g/L,DHA content 2.76%,DHA yield 0.29 g/L).The pathogenic bacteria and heavy metal levels in the algal oil were below the minimum limits specified in national food safety standard.[Conclusion]Using waste beer yeast hydrolysate obtained through ultrasound-assisted composite enzyme hydrolysis to cultivate Schizochytrium sp.for DHA production is feasible.This method achieves an organic integration of food production waste treatment and microalgae-based chemical product production.关键词
废啤酒酵母/水解液/裂壶藻/DHAKey words
waste beer yeast/hydrolysate/Schizochytrium sp./DHA引用本文复制引用
杨庆懿,唐裕芳,李涛,刘磊,周大科..啤酒废酵母水解液养殖裂壶藻生产DHA藻油[J].食品与机械,2025,41(2):173-180,8.基金项目
湖南省自然科学基金面上项目(编号:2024JJ7545,2022JJ90027,2021JJ30660) （编号:2024JJ7545,2022JJ90027,2021JJ30660）
