肉类研究2025，Vol.39Issue(4)：30-39,10.DOI:10.7506/rlyj1001-8123-20241016-264
不同贮藏温度下调理牛排菌群动态变化和相互作用
Dynamic Changes and Interactions of Bacterial Communities in Processed Beef Steak at Different Storage Temperatures
摘要
Abstract
In this study,we aimed to understand the bacterial diversity and interactions in processed beef steak at different storage temperatures.Comparative analysis of the bacterial community structure in chilled(4℃)and non-chilled(15℃)steak was performed,and the dynamic changes and interactions of bacterial communities in spoiled meat during storage at different temperatures after shelf life were analyzed using traditional microbial culture method and high throughput sequencing technology.The results showed that the total number of colonies was significantly lower at 4℃than that at 15℃(7.4×108 CFU/g versus 5.2×109 CFU/g)(P<0.001).The microbiota in steak was unaffected by storage temperature,but the dominant bacterial genera varied.Carnobacterium,Latilactobacillus and Pseudomonas were the dominant spoilage genera at both storage temperatures,Brochothrix being the only dominant genus at 4℃and Serratia at 15℃.The results of microbial community abundance correlation network analysis showed that the genera with the highest frequency of occurrence at PageRank>0.4 were Acinetobacter and Brochothrix.There was a correlation between the relative abundance of Acinetobacter and that of Serratia,Pseudomonas,and Brochothrix,between the relative abundance of Serratia and that of Brochothrix,and between the relative abundance of Brochothrix and that of Pseudomonas at a certain storage temperature and time,indicating a potential interaction between steak spoilage bacteria.Brochothrix,Latilactobacillus and Pseudomonas may be dominant spoilage bacteria in steak.关键词
贮藏温度/高通量测序技术/微生物多样性/种间相互作用Key words
storage temperature/high throughput sequencing technology/bacterial diversity/inter-species interactions分类
轻工纺织引用本文复制引用
史娜,李黎,邢超,宋丽萍,毛婷,杜建萍,李龙,耿健强,孙晓冬,杨丽梅..不同贮藏温度下调理牛排菌群动态变化和相互作用[J].肉类研究,2025,39(4):30-39,10.基金项目
北京市科技计划课题项目(Z221100007122005) （Z221100007122005）
北京市市场监督管理局科技计划项目(SJKJXM-2024-0004) （SJKJXM-2024-0004）
