肉类研究2025，Vol.39Issue(4)：11-16,6.DOI:10.7506/rlyj1001-8123-20241021-277
SinChERS净化-气相色谱-质谱法快速测定畜肉中吲哚乙酸
Rapid Determination of Indoleacetic Acid in Meat by Single-Step,Cheap,Effective,Rugged,and Safe(SinChERS)Purification Coupled with Gas Chromatography-Mass Spectrometry
摘要
Abstract
A method for the determination of indoleacetic acid in meat was established using a single-step,cheap,effective,rugged,and safe(SinChERS)purification and rapid derivatization followed by gas chromatography-mass spectrometry(GC-MS).After adding water and adjusting pH to 3 by formic acid,the samples were extracted with acetonitrile,purified on a SinChERS column,and blown to dryness under nitrogen.The residue was derivatized with concentrated sulfuric acid and methanol for 1 min prior to GC-MS analysis.Quantitative was performed using the matrix external standard method.The results showed that the linear relationship of indoleacetic acid concentration and its response value was good in the range of 0.01-5.0 μg/mL,with a correlation coefficient(r2)greater than 0.999.The limit of detection was 0.003 mg/kg and the limit of quantification was 0.01 mg/kg.The average recoveries of pork,beef,and mutton samples spiked at 0.01,0.05,and 0.10 mg/kg were 92.1%to 102.1%,with relative standard deviations(n=6)of 2.6%to 5.7%.The method is characterized by high sensitivity and short pretreatment time,and can be used for the determination of indoleacetic acid in meat.关键词
畜肉/吲哚乙酸/SinChERS/浓硫酸-甲醇/气相色谱-质谱法Key words
animal meat/indoleacetic acid/SinChERS/concentrated sulfuric acid-methanol/gas chromatography-mass spectrometry分类
化学化工引用本文复制引用
李霞,刘艳明,高喜凤,崔玉花,申中兰,陈倩倩,李芳芳,李海霞,徐大玮,李新玲..SinChERS净化-气相色谱-质谱法快速测定畜肉中吲哚乙酸[J].肉类研究,2025,39(4):11-16,6.基金项目
国家市场监督管理总局科技计划项目(2022MK072) （2022MK072）
