食品科学2025，Vol.46Issue(8)：114-123,10.DOI:10.7506/spkx1002-6630-20241012-069
冷藏黄鳝菌落演替规律及特定腐败菌致腐能力分析
Microbial Succession Pattern and Spoilage Potential of Specific Spoilage Bacteria in Refrigerated Monopterus albus
摘要
Abstract
To investigate the microbial succession and the spoilage potential of specific spoilage organisms(SSOs)in refrigerated Monopterus albus at 4℃,Illumina high-throughput sequencing was employed to analyze the diversity and dynamic shifts in microbial communities.Traditional selective culture methods were utilized to isolate and identify SSOs,and their spoilage potential was assessed by comparing the yield factor of spoilage metabolites(Y(TVB-N/CFU)).The results indicated that before storage(day 0),the microbial richness and diversity were the highest,with the relative abundance of Streptococcus(31.56%),Gemella(11.69%),Salinivibrio(7.5%),and Burkholderia-Caballeronia-Paraburkholderia(5.46%)being greater than 5%.During the middle and late stages of storage(≥15 days),Vagococcus,Acinetobacter,Burkholderia-Caballeronia-Paraburkholderia,and Psychrobacter emerged as the dominant genera.Linear discriminant analysis effect size(LEfSe)analysis revealed that the marker genera showing significant differences between before storage and after storage for≥15 days were primarily Streptococcus,Gemella,and Salinivibrio,Vagococcus,Acinetobacter,and Psychrobacter.Six SSOs were isolated from the 30-day stored sample,namely Pseudomonas fluorescens,Bacillus cereus,P.putida,Acinetobacter johnsonii,Lactococcus raffinolactis,and Carnobacterium divergens.The decreasing order of the Y(TVB-N/CFU)values of these SSOs was P.fluorescens(41.18 mg/CFU)>B.cereus(29.70 mg/CFU)>L.raffinolactis(15.07 mg/CFU)>A.johnsonii(14.59 mg/CFU)>P.putida(8.22 mg/CFU)>C.divergens(4.61 mg/CFU).This study provides a theoretical foundation for the development of efficient low-temperature preservation technologies for M.albus.关键词
黄鳝/冷藏/高通量测序/菌落演替/特定腐败菌/致腐能力Key words
Monopterus albus/refrigeration/high-throughput sequencing/microbial succession/specific spoilage organisims/spoilage potential分类
轻工业引用本文复制引用
廖鄂,杨贝,李涵,陈玉,张莹,杨倩,陈季旺..冷藏黄鳝菌落演替规律及特定腐败菌致腐能力分析[J].食品科学,2025,46(8):114-123,10.基金项目
湖北省重点研发计划项目(2023BBB111) （2023BBB111）
中央引导地方科技发展专项(2022BGE247) （2022BGE247）
湖北省乡村振兴科技支撑项目(2022BBA147) （2022BBA147）
农产品加工与转化湖北省重点实验室项目(2023HBSQGDKFA01) （2023HBSQGDKFA01）
