轻工学报2025，Vol.40Issue(2)：61-71,11.DOI:10.12187/2025.02.007
不同马尾松松针提取液的成分分析与抗氧化活性研究
Analysis and antioxidant activity study of different extraction liquid from Pinus massoniana needles
摘要
Abstract
To analyze the chemical composition and antioxidant activity of Pinus massoniana needles,the main components of essential oils,aqueous extracts,and alcoholic extracts were studied using gas chromatography-mass spectrometry(GC-MS)coupling method.Additionally,network pharmacology was utilized to investigate the active components,and free radical scavenging experiments were conducted to evaluate their antioxidant activities.The results revealed that 60 compounds,accounting for 95.13%of the total,were identified in the essential oils,primarily consisting of terpenes.The aqueous and alcoholic extracts contained 10 and 16 compounds,respectively,mainly consisting of ketones.Significant differences were observed in the composition distribution among different extraction methods.VIP analysis identified six potential candidate differential compounds:pinene(VIP=1.31),1-caryophyllene(VIP=1.47),L-β-pinene(VIP=1.80),α-pinene(VIP=3.32),3-nitro-1-phenylpropyl-1-ketone(VIP=7.86),and 2-ethoxyphenyl isothiocyanate(VIP=1.13).These compounds may exert effects on oxidative stress and free radical scavenging pathways through interactions with proteins such as aldehyde reductase(AR).In the evaluation of antioxidant activity,when the DPPH scavenging rate reached 80%,the mass concentrations of alcoholic extract,aqueous extract,and essential oil were 0.2 mg/mL,0.9 mg/mL,and 80 mg/mL,respectively.When the ABTS+scavenging rate reached 80%,the concentrations of alcoholic extract,aqueous extract,and essential oil were 0.07 mg/mL,0.2 mg/mL,and 10 mg/mL,respectively.When the hydroxyl radical scavenging rate reached 80%,the concentration of alcoholic extract was 3.5 mg/mL,while the scavenging abilities of aqueous extract and essential oil were relatively low and unstable.At the mass concentration of the extract liquid was 1.00 mg/mL,the alcoholic extract exhibited the strongest total reducing power.Therefore,Pinus massoniana needles possess significant antioxidant potential,with various components exhibiting antioxidant activities obtainable through different extraction methods.关键词
马尾松松针/提取方式/气相色谱-质谱(GC-MS)联用法/网络药理学/抗氧化活性Key words
Pinus massoniana needle/extraction method/gas chromatography-mass spectrometry(GC-MS)coupling method/network pharmacology/antioxidant activity分类
轻工纺织引用本文复制引用
刘亚群,陈良辉,张泽坤,郑玉忠,张振霞..不同马尾松松针提取液的成分分析与抗氧化活性研究[J].轻工学报,2025,40(2):61-71,11.基金项目
国家自然科学基金重点项目(31930004) （31930004）
广东省科技厅重点注设学科科研能力提升项目(20222DJ3070) （20222DJ3070）
潮州市科技局项目(202404GY06) （202404GY06）
